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Reference : UMR5239-ISASER-007
Workplace : LYON 07
Date of publication : Thursday, October 03, 2019
Type of Contract : FTC Technical / Administrative
Contract Period : 18 months
Expected date of employment : 2 December 2019
Proportion of work : Full time
Remuneration : Gross between 2 206 and 2 355
Desired level of education : 5-year university degree
Experience required : Indifferent
We study the impact of gene transcription on genome stability. In some regions of the genome, transcription interferes with DNA replication by producing DNA-RNA hybrids. The position of such hybrids and their direct impact on DNA replication remain poorly understood however. The candidate will map DNA-RNA hybrid forming regions upon DNA replication stress using several yeasts as model systems.
The candidate will optimize existing protocols to purify and sequence DNA-RNA hybrids and map such hybrids in several experimental setups.
We are looking for candidates with a strong expertise in the handling of nucleic acids. Previous experience with the preparation of sequencing libraries would be a bonus but is not essential.
The "Laboratoire de Biologie et Modélisation Cellulaire" (LBMC) is a research institute located at the "Ecole Normale Supérieure de Lyon" in the Gerland neighborhood of Lyon and hosts sixteen research teams. The institute aims at characterizing the molecular bases of cellular processes in normal and pathological conditions and develops genetic and quantitative analyses of biological systems and modelling approaches to do so. The project will be carried out in collaboration with the teams of Philippe Pasero (IGH, Montpellier, France), Sarah Lambert (Institut Curie, Orsay, France) and Chunlong Chen (Institut Curie, Paris, France) as part of a recently funded ANR proposal (Project ReDeFine).
Constraints and risks
It is possible that the candidate will have to spend short stays in the other teams of the research consortium.
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