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Portail > Offres > Offre UMR7216-BEARON-002 - CDD Ingénieur-e en épigénétique pour étudier la contribution de la chromatine à la réparation des dommages de la fourche de réplication (H/F)

Research engineer position to study the contribution of chromatin to the repair of replication fork damage

This offer is available in the following languages:
Français - Anglais

Date Limite Candidature : lundi 14 février 2022

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General information

Reference : UMR7216-BEARON-002
Workplace : VILLEJUIF
Date of publication : Monday, January 3, 2022
Type of Contract : FTC Technical / Administrative
Contract Period : 18 months
Expected date of employment : 1 May 2022
Proportion of work : Full time
Remuneration : Between 2 172 € and 2 293 € monthly gross salary based on experience
Desired level of education : 4-year university degree
Experience required : 1 to 4 years

Missions

An ATIP/Avenir funded CDD position is available in the laboratory of Dr. Beatrice Rondinelli at the Genome Integrity and Cancer Unit (UMR 9019, www.gustaveroussy.fr/en/umr-9019) of the Institut Gustave Roussy, located just outside of central Paris.
The laboratory studies the role of chromatin in the response to replication fork (RF) damage in human cells. The candidate will develop new cell systems and study the effect of specific mutations in the response to DNA damage, using cellular and imaging techniques to study DNA repair and genome stability.

Activities

Investigate the contribution of chromatin to replication fork (RF) damage repair. Using state-of-the-art genome editing approaches, the candidate will develop and utilize human cell lines that express a defined set of mutations recently identified in cancer in genes encoding chromatin components. In these systems, the candidate will study the contribution of the identified mutations in the response to replication fork damage by exploiting state-of-the-art imaging, proteomics, and cellular assays to study DNA repair and genome stability.

KEY ACTIVITIES:
- Generation of human cell lines encoding selected mutations in chromatin-encoding genes using CRISPR-based methods and other genome editing approaches
- Cellular and molecular characterization of generated mutant cells using state-of-the-art imaging, proteomics and cell-based assays to study DNA repair and genome stability.
- Assume part of the team's orders


Translated with www.DeepL.com/Translator (free version)

Skills

We invite applications from highly motivated and energetic individuals who are able to work independently and as a team player. The ideal candidate has a Bachelor +4/5 or Master degree, experience in cell/molecular biology in the field of cancer, hands-on experience in imaging techniques for the study of DNA repair markers and proteomics such as Western blot and immunoprecipitation. Additional experience in genomic editing approaches is appreciated. The contract is expected to start in May 2022.

Work Context

The host Genome Integrity and Cancers Unit UMR 9019 boasts a stimulating environment and dedicated equipment for research in the DNA repair and replication fields. Gustave Roussy Institute, the Leading Cancer Centre in Europe is the first best oncology hospital outside the United States and focused on the study of cancer biology and treatment. It offers access to state-of-the-art facilities including cell imaging, genomic, animal house and bioinformatics support. Initial funding is offered for a 18-months contract with possible extension.

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