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Reference : UMR7592-JULDUM-003
Workplace : PARIS 13
Date of publication : Thursday, June 25, 2020
Scientific Responsible name : Julien Dumont
Type of Contract : PhD Student contract / Thesis offer
Contract Period : 36 months
Start date of the thesis : 1 September 2020
Proportion of work : Full time
Remuneration : 2 135,00 € gross monthly
Description of the thesis topic
Chromosome alignment and segregation occur on a highly sophisticated spindle-shaped structure that is built from microtubules. In animal somatic cells and spermatocytes, microtubules are primarily assembled from the centrosomes. However, during the female meiosis of most animal species, chromosome segregation is preceded by a step of centrosome elimination. Much of our understanding of acentrosomal spindle assembly comes from experiments performed in cell-free Xenopus egg extracts. In this system, chromosomes act as a localized origin for spindle microtubules and several chromosome-linked molecular activities have been shown to be important for acentrosomal, spindle self-assembly. But how are these activities coordinated together and with microtubule motors and cross-linkers, to build a bipolar meiotic spindle in vivo in oocytes? By following three specific aims, the proposed project will provide a comprehensive and unprecedented view of the self-assembly mechanism and molecular players of acentrosomal spindles during meiosis I and II in the C. elegans oocyte. The candidate will take advantage of i) the host lab expertise in in utero live imaging, and correlative light and electron tomography of C. elegans oocytes, ii) the recent development of in vitro microtubule-based assays in the host lab, and iii) the in silico model that the host lab previously defined for the mitotic spindle, and that we will adapt here to meiosis.
The work will be carried out at the Institut Jacques Monod in Paris (FRANCE) in the Cell Division and Reproduction lab under the supervision of Julien Dumont
Constraints and risks
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