Missions

This post-doctoral project aims to determine the deregulation of DNA damage checkpoints in high-grade serous ovarian cancer (HGSOC) and differential sensitivity to corresponding pharmacological inhibitors, in relationship with their genetic contents (driver mutations), with the expectation to provide a predictive value.

Activités

- Generate stable non-transformed and HGSOC cancerous cell lines genetically modified and expressing DNA damage checkpoint sensors and various proteins in an inducible manner.
- Transiently express these sensors into patient-derived ovarian tumor cells (primary cultures).
- Participate in the development of a new FRET sensor (for details see Lebrec et al. Developmental Cell 57(5) 2022).
- Perform molecular and cell biology experiments (DNA cloning, cell culture, transfection and/or lentiviral infection, genome editing by CRIPSR/Cas9: Knock-Out and Knock-In).
- Perform video-microscopy experiments on living cells using functional perturbation assays (drugs).
- Quantitatively analyze cellular events on time-lapse video-microscopy image series.
- Data presentation: laboratory meetings, internal and external seminars in English.
- Report and article writing.

Compétences

- At least 3 years-experience in a research laboratory (excluding Master's internships) and a first-author scientific publication submitted or accepted.
- Autonomy and thoroughness in carrying out experiments.
- Excellent organizational skills.
- Solid skills in molecular and cell biology: DNA cloning, culture and maintenance of mammalian cell lines, transfection/infection, genetic modification by CRISPR/Cas9.
- Fluorescence microscopy skills (on fixed and/or live cells).
- Experience in biological image analysis.
- Confident to speak in English.
- Good interpersonal skills and ability to work as part of a team.

Contexte de travail

Our team is part of the UMR9019 CNRS research unit "Genome Integrity and Cancer" located at the Institut Gustave Roussy - Université Paris-Saclay, a leading cancer research center in Europe (~20 min from downtown Paris). The Institute has various on-site platforms for live-cell imaging (video, confocal, spinning disk, super-resolution microscope), intravital imaging in animals, flow cytometry, bioinformatics and genomics. The successful candidate will benefit from a stimulating scientific environment with several research groups working on DNA integrity and replication/repair mechanisms.

Contraintes et risques

Lentiviral infection will be carried out in the P3 containment laboratory.
our institute provides mandatory training for access to and use of the P3.