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Portail > Offres > Offre UMR7592-BENLAD-007 - H/F Post Doc Organisation des organoides intestinaux à l'interface entre biologie cellulaire et mécanique

H/F Post Doc Mechanics and organzation of intestinal organoids.

This offer is available in the following languages:
Français - Anglais

Date Limite Candidature : vendredi 3 juin 2022

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General information

Reference : UMR7592-BENLAD-007
Workplace : PARIS 13
Date of publication : Friday, May 13, 2022
Type of Contract : FTC Scientist
Contract Period : 24 months
Expected date of employment : 1 October 2022
Proportion of work : Full time
Remuneration : from 2 750 € gross according to experience
Desired level of education : PhD
Experience required : Indifferent

Missions

This position is at the interface between cell biology and biophysics at the IJM (Paris). It will include collaboration with theoretical physicists. The aim of the project is to understand interplay between mechanical forces, cell extrusion and cell behaviours during the self-organization of intestinal organoids. Intestinal organoids have been proven to capture essential features of the intestinal epithelium such as crypt folding, cellular compartmentalization and collective dynamics. We will focus on the role of extruding cells in the regulation of the homeostatic behavior of intestinal organoids. These behaviours are likely coordinated by mechanosensitive processes, although the impact of mechanics remains to be elucidated.
We will investigate specific mechano-transduction processes that link mechanical stresses and cell behaviours including intestinal differentiation and the cell fate of extruding cells.
Integrating mechanics, engineering and cell signaling approaches across scales from sub-cellular, cellular and multicellular levels, we aim at understanding the physical principles and molecular mechanisms that control cell extrusion processes. For this, we will use a combination of experiments including imaging, micromechanics and microfabrication to investigate the role of mechanics in controlling the self-organization of intestinal organoids.
We will particularly focus on the role of the acto-myosin cytoskeleton in cell extrusion mechanisms focusing on the extruded cell and its immediate neighbors (stable organoids that express actin or myosin markers) in correlation with caspase activity. We will analyze cells under tensile or compressive stresses together with shape descriptors and stress measurements.

Activities

The successful candidate will :
- participate in the design of the project,
- use and develop new methodologies,
- carry out the experiments,
- analyze and communicate the results,
- write reports and research papers.
The candidate will use a wide range of approaches at the interface of cell biology and mechanics, including advanced live cell microscopy, cell biology (mammalian cell culture, transfection, molecular biology...) and micromanipulation and microfabrication to control and measure the dynamic properties of intestinal organoids.

Skills

The candidate must hold a PhD (or have obtained it before the beginning of the contract), in biology or biophysics with a strong biology component. The candidate must be able to work independently but also in a team in the framework of a collaborative project with teams outside the institute, have the ability to lead a project, and must be able to express himself/herself in English, written and spoken (French is not mandatory).

Work Context

The Institut Jacques Monod (IJM), housed in a 1800 square meter building, located on the new campus of the urban project Paris rive gauche, is one of the main centers for basic research in biology in Paris, and offers a multidisciplinary research environment. The IJM brings together some thirty research groups working on cell and developmental biology, biophysics and genomics. The postdoctoral researcher will benefit from a highly stimulating scientific environment, and will have access to state-of-the-art equipment (notably the photonic microscopy, electron microscopy and flow cytometry platform), located at the IJM.

Constraints and risks

Not relevant

Additional Information

The contract is for a fixed term funded by the ERC Advanced Dead or Alive with the possibility of extension. The position is to be filled starting in September 2022. Applications should include a CV and cover letter summarizing the applicant's scientific interests, and contact information for two scientists who can provide recommendations.

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