Missions

The recruited postdoctoral researcher will be responsible for developing murine cardiac organoid models, building on previous work conducted in the lab. In particular, the goal will be to improve our understanding of the migration and differentiation of initially present stem cells, in response to chemical and mechanical stimuli. The project will be carried out on-site in Charles Baroud's team at the Institut Pasteur.

Activités

The project will be primarily experimental, involving the culture of mouse embryonic cells under conditions that promote their differentiation into cardiac organoids. The work will begin with cell culture under varying conditions, followed by quantitative imaging of various cell type markers to understand the emergence of different phenotypes. These microscopy-based measurements will be combined with RNA sequencing and other analytical techniques. Finally, modifying the mechanical environment will be used to probe the response of these cells during organoid development.

Compétences

The candidate must hold a PhD in biology, biophysics, or a related field. Skills in cell biology and biomaterials are important, as well as experience in confocal microscopy. A background in image analysis is a plus.

Contexte de travail

Access to the experimental facilities of Charles Baroud's lab at the Institut Pasteur, including cell culture, microscopy, and computational analysis tools. The researcher will also have access to the core facilities of the Institut Pasteur.

Contraintes et risques

N/A

Informations complémentaires

ANR collaboration between Charles Baroud (Institut Pasteur) and Hervé Turlier (CNRS, Collège de France)