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Reference : UMR6230-JEADUM-007
Workplace : NANTES
Date of publication : Monday, May 18, 2020
Scientific Responsible name : Jean-Nicolas Dumez
Type of Contract : PhD Student contract / Thesis offer
Contract Period : 36 months
Start date of the thesis : 1 October 2020
Proportion of work : Full time
Remuneration : 2 135,00 € gross monthly
Description of the thesis topic
NMR spectroscopy is an essential source of information in chemistry, to characterize the structure and dynamics of molecules. The time scales available for real-time NMR analysis are however limited by the time required to record information-rich two-dimensional (2D) data. “Spatial coding”, a parallelization approach inspired by MRI, allows 2D experiments to be accelerated by a factor of 1000, and the resulting “ultra-fast” experiments thus open up many opportunities for studying out-of-equilibrium chemical systems. The NMR team of the CEISAM laboratory is among the international leaders in the field of NMR analysis of mixtures, and recent developments in the laboratory have already made it possible to increase the field of application of methods based on spatial coding for online monitoring and hyperpolarization [1-2]. The laboratory is equipped with state-of-the art instrumentation, such as a prototype for dynamic nuclear polarization (DNP).
The objective of this project is the development and application of new NMR methods exploiting the concept of spatial coding, for the ultrafast analysis of out-of-equilibrium samples. It targets more specifically the development of ultrafast 2D correlations experiments (DOSY, multi-quantum), which can be applied for the online monitoring of chemical reactions, and for the analysis of hyperpolarized substrates. The project involves the development of
• methods allowing the use of spatial encoding on a flowing sample, for online monitoring of chemical reactions. These methods will be used for flow chemistry and structural elucidation by online analysis;
• methods exploiting spatial coding for the analysis of hyperpolarized substrates by DNP, for the analysis of samples of mixtures of small molecules with low concentrations.
These two axes will use the instruments recently installed in the CEISAM laboratory: a DNP polarizer, and a high-field online monitoring system, associated with an NMR probe equipped with high-performance magnetic field gradients. The project will therefore include a strong experimental component, which will guide the development of new NMR methods.
The work will be carried out in a stimulating collaborative environment, with many other doctoral students and post-doctoral students recently recruited as part of two ERC projects. The recruited doctoral student will interact more specifically with several doctoral students and post-doctoral students, as well as with several specialists in the development of pulse sequences.
CEISAM is the molecular chemistry laboratory of the University of Nantes and brings together 5 recognized research teams in theoretical, physical and analytical chemistry, and in organic synthesis. The CEISAM laboratory NMR platform is the largest NMR platform in Western France. Its equipment includes 6 high field spectrometers (400 - 700 MHz), 3 low field spectrometers, equipment for MRI and a polarizer for nuclear dynamic polarization. CEISAM is located in the dynamic environment of the city of Nantes, near the Atlantic coast and South Brittany.
1. J.-N.Dumez et al., Hyperpolarized NMR of plant and cancer cell extracts at natural abundance. Analyst 140, 5860 (2015)
2. C. Jacquemmoz, F. Giraud, and J.-N. Dumez, Online reaction monitoring by single-scan 2D NMR under flow conditions, Analyst 145, 478 (2020).
The candidate has a background in chemistry (preferably physical or analytical chemistry) or in physics, and an interest in the development of analytical methods through an approach combining theory and experiments. Due to the collaborative and international nature of the project, good communication skills in English are required. The recruited doctoral student will be required to transmit his knowledge to other students (Master, Doctorate) and to present his work at an international congress.
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