Informations générales
Intitulé de l'offre : PhD position in structural biology (M/F) (H/F)
Référence : UMR5075-VALLAN-039
Nombre de Postes : 1
Lieu de travail : GRENOBLE
Date de publication : mardi 13 mai 2025
Type de contrat : CDD Doctorant
Durée du contrat : 36 mois
Date de début de la thèse : 1 septembre 2025
Quotité de travail : Complet
Rémunération : 2200 gross monthly
Section(s) CN : 22 - Biologie cellulaire, développement, évolution-développement, reproduction
Description du sujet de thèse
The PhD topic will be to identify the competition mechanism for bacterial peptidoglycan biosynthesis along the cellular cycle, in particular thanks to the study of Mur chimeric enzymes that are naturally present in certain bacterial genomes.
The student will use molecular biology techniques, biochemistry, X-ray crystallography and confocal and fluorescence microscopies.
Activities :
- Produce chimeric proteins implicated in the biosynthesis of the bacterial peptidoglycan precursors.
- Solve the three-dimensional structure of the chimeric proteins.
- Study the implication of mutations on the peptidoglycan recruitment during the different phases of the cellular cell cycle by confocal and fluorescence microscopies.
Skills :
- Knowledge in molecular biology, biochemistry and recombinant proteins purification
- Interest for structural biology is required
- English (written/oral) level C1
- Capabilities to work independently in a multidisciplinary environment.
Contexte de travail
Located on the Grenoble Science Park (ESRF/ILL/EMBL/IBS), the Institute of Structural Biology (IBS) is a national and international player in the field of integrated structural biology. The IBS is a research center, a technical platform, a host site and a scientific training center. Its mission is to develop research in structural biology, a field of research that is crucial to the understanding of fundamental biological mechanisms. It is supported by 12 state-of-the-art platforms.
As a joint research unit (CEA-CNRS-UGA), the IBS is composed of 20 research groups, each of which proposes a multi-disciplinary approach, at the frontiers of biology, physics and chemistry, in coherence with 3 research axes. Nearly 320 people work there as researchers, PhD students, engineers and technicians in a multicultural and international environment.
The person recruited will join the Pneumococcus group at IBS (https://www.ibs.fr/en/research/microbiology-infection-and-immunity/pneumococcus-group-c-morlot/?lang=en)
Contraintes et risques
- The research will be carried out in either French or English, however it is expected that the non-French speaking candidate will strive to learn the basics of French in order to facilitate communication and integration into the laboratory.
- Teleworking will only be permitted during the thesis writing period.