Missions

Our lab focuses on understanding how cell identity and epigenetic memory are sustained or altered during cell division in B lymphopoiesis. We use innovative technologies that combine mitotic chromosome flow-sorting with multiomic approaches - proteomics, chromatin RNA-seq, and chromatin accessibility - to analyse native (unfixed) mitotic chromosomes.
Our goal is to identify DNA-binding factors and epigenetic 'bookmarks' that remain chromatin-associated during mitosis and enable reactivation of transcriptional programs in daughter cells. 

This ATIP-Avenir (CNRS)-funded postdoctoral project focuses on X chromosome inactivation (XCI), a paradigm of epigenetic inheritance, and its link to female-biased immune responses. The X chromosome is enriched in immune-related genes, and stable maintenance of XCI is increasingly recognized as essential for immune regulation. The project aims to (1) define the mitotic proteome of active and inactive X chromosomes in human B lymphoid cells, (2) identify candidate factors involved in XCI mitotic memory, and (3) investigate their role in regulating X-linked immune gene dosage, with potential implications for sex-biased immunity.

Activités

• Mitotic chromosome sorting by flow cytometry
• Implementation of multiomic approaches: proteomics, epigenomics, ATAC-seq, RNA-seq
• Data analysis in collaboration with bioinformatics facility
• Validation and functional characterization of chromosome-bound candidate factors using CRISPR/degron strategies and nascent transcriptional analysis
• Presentation of results in lab meetings, seminars, and conferences
• Manuscript writing and contribution to the scientific dissemination of the project

Compétences

• Strong background in molecular and cellular biology
• Solid background and scientific interest in epigenetics, cell differentiation, and hemato/immunology
• Experience with at least one omic approach (ATAC-seq, RNA-seq, ChIP, etc.)
• Skills in cell culture and handling of stem cell lines and primary cells
• Scientific rigor, critical thinking, autonomy, and strong team spirit
• Proficiency in English (spoken and written)
• At least one first-author publication is highly desirable

Contexte de travail

The successful candidate will join a dynamic, collaborative lab within a network of six groups at the Epigenetics and Cell Fate (EDC) Centre. Our Institute is located in central Paris and offers access to excellent core facilities in proteomics, flow cytometry, imaging, genome engineering, and bioinformatics, in partnership both at the EDC and the Institut Jacques Monod.

Contraintes et risques

• Handling of human cells and biological material
• Potential exposure to standard laboratory chemicals
• Occasional out-of-hours work may be required depending on experimental needs
• Strict adherence to laboratory safety regulations, including protocols in confined (Level 2) environments