Faites connaître cette offre !
Reference : UMR5306-THODEH-001
Workplace : VILLEURBANNE
Date of publication : Tuesday, February 16, 2021
Type of Contract : FTC Scientist
Contract Period : 17 months
Expected date of employment : 10 May 2021
Proportion of work : Full time
Remuneration : 3000€
Desired level of education : PhD
Experience required : Indifferent
Background: The mechanical properties of cells are altered during tumor transformation and in response to drugs. Monitoring these parameters could offer label free tools to evaluate new therapeutic strategies and provide new indicators to guide clinicians in the personalization of therapy. For these applications, it is crucial to probe the mechanical properties of 3D tumor models in vitro. However, available microscopy techniques provide contrast based on optical properties which cannot reveal mechanical properties. In addition, the use of fluorescent probes alters normal cellular functions and cannot reveal an efficiency gradient in 3D tissues.
Project: Brillouin microscopy (BLS) is a non-contact imaging technique that does not require the use of contrast agents, exploiting the infinitesimal natural vibrations of matter. We recently applied BLS to organoids composed of two cell lines of different metastatic grades: we showed that we could distinguish them by their mechanical properties. We also followed the local variations of the mechanical properties during drug treatment: the center of the tumor remains rigid longer than the edge, demonstrating a gradient of effectiveness of the treatment. Based on these results, we want to design a quantitative, label-free 3D imaging platform to 1) track the progress of anti-cancer drugs and 2) demonstrate the ability of BLS to identify tumor stage by its mechanical properties.
-development of a user-friendly imaging platform based on Brillouin spectroscopy.
-proof of concept experiments to demonstrate the ability to assess the stage of basic functional histopathological units of the breast.
-data analysis, in close collaboration with our partners in biology and pharmacy.
-participation in the preparation of microfluidic devices.
-participation in other laboratory projects, especially in laser ultrasound
-contribution to student mentoring.
-work experience at the physical / biology interface
-programming with Labview
-data analysis with Matlab and COMSOL
The research will be carried out at the Institut Lumière Matière, in the biophysics team. It will be done in collaboration with the Lyon Cancer Research Center and LAGEPP
We talk about it on Twitter!