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Reference : UMR5075-JERGAU-003
Workplace : GRENOBLE
Date of publication : Thursday, January 07, 2021
Type of Contract : FTC Scientist
Contract Period : 24 months
Expected date of employment : 1 April 2021
Proportion of work : Full time
Remuneration : According to experience and from 2437 € gross
Desired level of education : PhD
Experience required : Indifferent
Recent advances in cryo-electron microscopy open up exciting scientific perspectives in the understanding of molecular mechanisms in biology.
In this context, the IBS, together with the ISBG, has established a user platform dedicated to electron microscopy, equipped with state-of-the-art equipment, including a high-resolution Glacios cryo-electron microscope (equipped with two direct electron detectors). The current opening aims to recruit a research engineer (IR) in the Electron Microscopy and Methods (MEM) group of the IBS to maintain and operate this high-performance microscope.
The IR will operate the equipment of the IBS-ISBG cryo-electron microscopy platform in connection with the national infrastructure FRISBI (French Infrastructure for Integrated Structural Biology) and European infrastructure Instruct-ERIC (the European infrastructure for Integrated Structural Biology). He/she will carry out routine procedures and developments in sample preparation, data acquisition and data processing for cryo-electron microscopy (cryo-EM) studies. Playing a central role within the platform, the IR will guarantee the proper use of the equipment by accompanying and training its users and will be responsible for its maintenance.
Operation of the instruments
- Operate and manage the high-resolution Glacios cryo-electron microscope.
- Define, test and set up procedures to facilitate the monitoring and standardization of experiments.
- Optimize experimental conditions (cryo-EM screening of samples, high-resolution optimization of the microscope) on a case-by-case basis for new samples
- Participate, when necessary, in the operation of the 2 other electron microscopes (T12 and F20) of the platform.
- Develop, set up and update automatic data processing "pipelines".
- Set up remote access for the Glacios EM.
Training and supervision
- Supervise and train users in the use of the instruments (primarily the Glacios, but also T12 and F20) and image processing.
- Advise users on methodological approaches, sample preparation and image analysis/3D reconstruction/refinement of structures in cryo-EM
- Participate in the implementation and leading of training initiatives (workshops, continuing education)
- Drafting of technical notes
Maintaining the instrumentation at the best level and new developments
- Interact with manufacturers and monitor/validate the maintenance of the equipment.
- Participate in the basic maintenance of the microscopes and related instruments (glow discharger, carbon evaporator and Vitrobot).
- Ensure a technological and scientific overview of the field of electron cryo-EM for biological applications (single particle analysis, cryo-electron tomography).
- When necessary, coordinate and implement the use of new cryo-EM techniques or new instruments.
- Communicate technological results and achievements in the form of reports, patents, publications, oral presentations and teaching.
- Participate in the research projects of the host team and users
- Possibility of developing your own method development-oriented research activity in discussion with your supervisor..
- Become rapidly autonomous and operational as an expert in high-resolution cryo-EM. An existing masteryof the latest generation of electron microscopes will be a plus.
- Mastery or theoretical knowledge of at least one of the image acquisition software for single particle analysis or electron tomography (e.g. TFS EPU, Digital Micrograph, SerialEM etc.).
- One or more of the following skills is advantageous:
- Good knowledge of the different approaches used in electron microscopy (single particle analysis of frozen-hydrated complexes, tomography or diffraction)
- Knowledge of the different methods of sample preparation (negative staining, vitrification, cellular electron microscopy)
- Curiosity, desire to develop new skills and to invest in the booming field of cryo-EM.
- Scientific English level C1 according to the Common European Framework of Reference for Languages
- Ability to manage different projects in parallel and to arbitrate between priorities and contingencies, respecting projectdeadlines
- Ability to interact and work in a team and with diverse audiences (interdisciplinary and multicultural)
- Proven pedagogical skills
- Autonomy and sense of initiative
Located on the EPN site (ESRF/ILL/EMBL/IBS) in an international environment on the Scientific Campus of Grenoble, the Institute of Structural Biology (IBS) is a joint research unit of 280 people, under the supervision of CNRS, UGA and CEA. The IBS is part of the Partnership for Structural Biology (PSB), which brings together the structural biology components of ESRF and ILL as well as EMBL and IBS and allows for daily scientific exchange among the four institutes.
As a research center, a technical platform, and a scientific training site all at once, the IBS is dedicated to research and development in structural biology, a field of research that is crucial to the understanding of fundamental biological mechanisms.
The research activities of the IBS are structured into 3 axes and 19 research groups. These groups are supported by a state-of-the-art research infrastructure that includes 12 platforms.
The IR to be recruited will be assigned to the Instruct-ERIC/FRISBI platform of the IBS-ISBG (5 people), which is supported by the Electron Microscopy and Methods group of the IBS (20 people). The platform is equipped with 3 microscopes: a Glacios with direct electron detectors, a screening cryo-electron microscope that is also used for electron tomography (Tecnai F20) and a microscope for negative staining (T12). The platform is also equipped for the preparation of samples for analysis by cryo-EM (grid preparation and freezing systems). Cryo-EM is a technique of choice for the analysis of structure and dynamics of biological complexes/nanomachines, with many technological developments and new approaches to be integrated in the future.
Within the platform and under the direct authority of its Scientific Director, he/she will interact on a daily basis with the other engineers and technicians in the platform.
Initial duration of the contract is 24 months but may be extended.
Constraints and risks
The engineer will work on a normal weekday schedule but may very occasionally be required to make short interventions during the weekend.
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