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Reference : UMR5075-BRUFRA1-001
Workplace : GRENOBLE
Date of publication : Monday, November 25, 2019
Type of Contract : FTC Scientist
Contract Period : 24 months
Expected date of employment : 1 February 2020
Proportion of work : Full time
Remuneration : 2617.05 to 3017.31 € /month
Desired level of education : PhD
Experience required : 1 to 4 years
The mission is part of an ANR research program focusing on the understanding of proteasome regulation mechanisms (period covered: 2019-2022). The candidate, biochemist and / or structuralist training, will highlight the role of new proteins in the recruitment and functional coordination of different machineries partners proteasome, to promote a recycling of defective ribosomes in hyperthermophilic archaea .
- Purification of native and recombinant supramolecular complexes.
- Sample preparation for electron microscopy and crystallography.
- Functional and biophysical characterization of complexes in solution
- Structural studies in crystallography and electron microscopy.
The candidate must have a background in biochemistry and structural biology with an interest in large cellular machineries and extremophile biology. We are looking for skills in the study of native and recombinant supramolecular complexes. Experience in structural biology (crystallography and / or electron microscopy) is desired.
The IBS is a research unit, affiliated to Grenoble-Alpes University, CNRS and CEA. It currently employs 280 people, including many students and postdocs from different countries. It has efficient and internationally competitive facilities in molecular and cellular biology, biochemistry, biophysics and structural biology. The Institute is a founding member of the Partnership for Structural Biology with prestigious pan-European institutes: the European X-synchrotron source, the European neutron scattering center, the EMBL Grenoble station, all located nearby and offering a strong international environment. In this context, the ELMA group (website: http://www.ibs.fr/research/research-groups/group-extremophiles-and-large-molecular-assemblies-b-franzetti/) specializes in structural biology of archaeal cell machineries with a focus on intracellular proteolysis. The laboratory is developing specific tools in crystallography and small angle scattering for the study of large assemblies. The proposed subject is part of a collaboration with IFREMER in Brest and with the microscopy team of the IBS, which are partners of the ANR PROTE-IN project (period covered: 2019-2022). Supervision will be provided by a DR researcher. The competences present in the ELMA group and the access to the panel of biophysical characterization platforms of the IBS will allow the candidate to acquire a solid expertise in integrative structural biology
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