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Reference : UMR8612-FRAFAY-002
Workplace : CHATENAY MALABRY
Date of publication : Monday, September 13, 2021
Scientific Responsible name : Elias Fattal, Francois Fay
Type of Contract : PhD Student contract / Thesis offer
Contract Period : 36 months
Start date of the thesis : 1 November 2021
Proportion of work : Full time
Remuneration : 2 135,00 € gross monthly
Description of the thesis topic
The goal of the research proposal is to develop a new strategy to deliver siRNA using nanoparticles functionalized by aptamers to induce reprogramming of Tumor-Associated Macrophages. This project will be caay out within the group of Prof. Elias Fattal at Institut Galien Paris-Saclay in collaboration with Dr. Jean-Luc Perfettini at the Institut Gustave Roussy.
Aptamers (Apts) consist of short, single-stranded DNA or RNA oligonucleotides with demonstrated high affinity toward specific targets. Unlike antibodies, Apts are typically selected in vitro from combinatorial oligonucleotide libraries by a high-throughput process known as Systematic Evolution of Ligands by Exponential Enrichment (SELEX). In the last few years our laboratory has developed new Aps-conjugated nanoparticles that can target specific cells both in vitro and in vivo3–6. Using this expertise, we plan to develop new aptamer-conjugated nanoparticles to target Tumor-Associated Macrophages and deliver siRNA to induce macrophage reprogramming.
The specific objectives of this work are as follows:
(i) Selection of aptamers targeting Tumor-Associated Macrophages using SELEX.
(ii) Development and physicochemical characterization of Apts-NPs encapsulating SiRNA.
(iii) Evaluation of the NPs in vitro & in vivo.
For this project, we are seeking a candidate with a pharmacy, engineering school or MSc degree, or equivalent in Pharmaceutical science, Biotechnology or Biochemistry willing to evolve towards the chemistry/biology interface.
The Institut Galien Paris-Saclay is a multi-disciplinary research institute specialized in biomedical nanotechnology. The institute is considered a key player in the nanomedicine field in both France and Europe with the filing of 15 patents and the creation of 3 start-up companies within the last 5 years. The student will benefit from the technical support of dedicated personnel and equipment ranging from chemical synthesis, physical-chemistry analysis, radioactivity, cell culture to animal experiments. As part of our diversity commitment, we are welcoming and integrating people with disabilities.
Constraints and risks
Work related to biochemistry, nanoscience, and in vitro / in vivo biology with the standard requirements for these fields of work (ventilated fume hood, personal protective equipment, etc).
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