Reference : UMR7361-ISABRI-001
Workplace : MULHOUSE
Date of publication : Friday, September 23, 2022
Scientific Responsible name : Isabelle Brigaud
Type of Contract : PhD Student contract / Thesis offer
Contract Period : 36 months
Start date of the thesis : 1 March 2023
Proportion of work : Full time
Remuneration : 2 135,00 € gross monthly
Description of the thesis topic
PhD project Summary
Since their first use in 1962, silicone implant biocompatibility is challenged by the presence of silicone liquid droplets and platinum catalyst permeating from the gel filling through the silicone elastomeric shell then migrating to the host periprosthetic tissue. Periprosthetic tissue refers to the fibrotic capsule developed around the implant as a result of the foreign body reaction (FBR) process. In this context, the candidate will be in charge 1) to select and to characterize starting silicone materials. 2) To evaluate which permeating species negatively impact FBR by studying interaction of silicone materials with (i) protein (fibrinogen) and (ii) immune cells (macrophages). (3) to test biological performances of the innovative medical silicone implant prototype engineered after knowledge-based generated in (1) and (2). Together, this research project will allow the selected candidate to acquire an interdisciplinary spectrum of skills in medical biointerfaces, silicone chemistry, biochemistry, cellular and molecular biology and confocal imaging.
The PhD is essentially experimental and relies on interdisciplinary approaches.
The student will have to:
• select and characterize model of medical grade silicone filling and membrane
• develop a protein assay to evaluate impact of silicone/Pt onto protein adsorption kinetics and conformation
• develop a macrophage assay in order 1) to screen the toxicity degree of permeating silicone/Pt species and 2) to evaluate parameters priming chronic inflammation
• translate biological assays to test the final prototype.
• Analyze and interpret the results
• Communicate on the results (oral & written)
• Collaborate with researcher & engineer
• Potential candidate should demonstrate a strong interest in interdisciplinary approaches
• Candidates must possess Master's degree or equivalent with strong background in cell culture (macrophage cell lines), molecular biology (qPCR, Western blot, Elisa), protein biochemistry and/or material biointerfaces (cell/material interaction) and confocal imaging (immunostaining and imaging)
• Candidates with knowledge on (i) macrophage cell culture, (ii) FBR and/or (iii) fibrinogen will be considered in priority
• Self-motivated person, scientific creativity and originality, strong team spirit, collaborative capacity, excellent time management, rigor, perseverance, and writing skills
• Fluency in English is essential (B2 level minimum)
Applicants must send the required documents. Documents in English will are recommanded
• a full CV
• a motivation letter including a description of previous research experiences and contact details, official transcript. Please indicate the ranking of the project based on your preference if you have applied elsewhere (first choice, second choice...)
• at least one recommendation letter
Applicants should submit before December 15th the documentation.
Shortlisted candidates will be invited for in-person interviews (on site or by videoconference). We will endeavour to provide feedback to unsuccessful applicants where possible.
• A highly valuable expertise in medical biointerfaces, silicone chemistry, biochemistry, cellular and molecular biology.
• Interdisciplinary supervision. The PhD student will be supervised by 2 or 3 academics from different disciplines: Polymer and Biology.
• industrial and academic secondments
PhD fully funded by the French National Research Agency (ANR) in the field of technology for health,
is available in the Biomaterials-biointerface group at CNRS IS2M (Institut de Science des Matériaux; https://www.is2m.uha.fr/fr/nos-axes-thematiques/biomateriaux-biointerfaces/ Mulhouse, France (group leader Karine Anselme)). The successful candidate will join highly dynamic research environment and get access to several core facilities and relevant state-of-the-art technologies. She/he will benefit from international conference attendance and opportunities to collaborate with the research-intensive SME STATICE (Besançon, France; http://www.statice.com/fr) specialized in the engineering and design of medical silicone devices and polymer physicists at the European Institute of Membrane (IEM, Montpellier; http://www.iemm.univ-montp2.fr/).
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