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Reference : UMR7277-FLOBES-009
Workplace : NICE
Date of publication : Tuesday, December 22, 2020
Scientific Responsible name : Florence Besse
Type of Contract : PhD Student contract / Thesis offer
Contract Period : 36 months
Start date of the thesis : 1 March 2021
Proportion of work : Full time
Remuneration : 2 135,00 € gross monthly
Description of the thesis topic
Our main research goal is to understand how post-transcriptional regulatory processes control neuronal plasticity in vivo. Specifically, we study how neuronal ribonucleoprotein (RNP) granules, which are dynamic condensates enriched in RNAs and regulatory proteins, are regulated in space and time to promote the local remodeling of neuronal cells during development, as well as in learning and memory processes. Although alterations in the properties of neuronal RNP granules have been associated with different neurodegenerative diseases, surprisingly little is known about the molecular mechanisms underlying their formation and dynamics, as well as their physiological function and regulation. To address these questions, we use Drosophila (fruit fly) nervous system as a powerful in vivo system where advanced genetics, imaging and biochemistry can be combined. Over the years, we have used and developed a range of complementary assays including in vivo live-cell imaging, in vivo RIP-seq, high throughput-microscopy, in vitro reconstitution assays. For some of our projects, we also collaborate with computer scientists to perform automated and quantitative image processing.
The successful candidate will specifically investigate the response of neuronal RNP granules to physiological aging. His/her project is based on recent discoveries from the lab indicating age-dependent and population-specific changes in the properties and functions of RNP granule properties.
The Institute of Biology Valrose (iBV) is an international Institute hosting 26 research groups addressing fundamental biological questions on a wide range of biological models. It hosts state-of-the-art technological platforms for microscopy, cytometry and bioinformatics. English is the working language in the lab and in the Institute.
Constraints and risks
Adaptation of working hours to experimental procedures
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