Reference : UMR7249-HERRIG-010
Workplace : MARSEILLE 13
Date of publication : Monday, June 20, 2022
Scientific Responsible name : Hervé Rigneault
Type of Contract : PhD Student contract / Thesis offer
Contract Period : 36 months
Start date of the thesis : 1 November 2022
Proportion of work : Full time
Remuneration : 2135 euros bruts
Description of the thesis topic
TITLE: Vibrational speckle tomography microscopy for fast intra-operative cancer tissue histopathology
Histology is commonly practiced in hospitals and consists of taking a small sample of tissue (biopsy) which is sliced and stained to be finally observed by a specialist allowing him to make a diagnosis on its cancerous nature. This process is long (1h-24h) and prevents the optimal management of patients during surgery or endoscopic exploration. This PhD research project aims at developing a completely new optical technology that allows instantaneous three-dimensional histological imaging of biopsies without the use of any label or preparation. The proposed approach uses wide-field coherent Raman imaging using random illumination (speckle) and reconstruction algorithms to reconstruct a three-dimensional image of the chemical bonds present in the sample, without any labeling or preparation. This approach closely links the latest technological innovations in terms of laser, microscopy and low noise optical detection with advanced digital reconstruction methods developed.
The proposed work is related to the recently published paper [1,2] by the Mosaic group at the Fresnel Institute that demonstrate numerically coherent Raman ptychography and tomography. The goal of the PhD work is to implement these ideas experimentally and to apply them to 3D histology of ex-vivo tissues.
 S. Heuke, H. Rigneault, and A. Sentenac, "3D-coherent anti-Stokes Raman scattering Fourier ptychography tomography (CARS-FPT)," Optics Express 29, 4230-4239 (2021).
 S. Heuke, K. Unger, S. Khadir, K. Belkebir, P. C. Chaumet, H. Rigneault, and A. Sentenac, "Coherent anti-Stokes Raman Fourier ptychography," Opt. Express 27, 23497-23514 (2019).
This work is linked to the ERC project SPECKLECARS
The work will take place in a fully equipped optical room with innovative lasers and state-of-the-art opto-electronic equipment.
Constraints and risks
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