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Reference : UMR7249-GUIBAF-005
Workplace : MARSEILLE 13
Date of publication : Tuesday, July 21, 2020
Scientific Responsible name : Guillaume Baffou
Type of Contract : PhD Student contract / Thesis offer
Contract Period : 36 months
Start date of the thesis : 1 October 2020
Proportion of work : Full time
Remuneration : 2 135,00 € gross monthly
Description of the thesis topic
Quantitative phase imaging and laser heating of bacteria using gold nanoparticles
Life on Earth exists up to temperatures of 113 ° C, especially concerning microorganisms that live at the bottom of the oceans. If it is possible to fetch them, it is however difficult to observe them alive, because no commercial optical microscope operates at such temperatures.
The thesis project consists of developing a new type of optical microscope capable of studying hyperthermophilic microorganisms. The approach is based on laser heating of gold nanoparticles, and monitoring by quantitative phase microscopy. The objective will be to get to see hyperthermophilic organisms live by light microscopy, and to learn more about their metabolism, their interactions, their life.
The experiments will mainly consist in cultivating cells, and working on a "home-made" optical microscope. Most of the work will remain experimental optics. We are therefore looking for a student with a solid background in experimental physics, wishing to work at the optical / biology interface.
The thesis work will be carried out at the Fresnel Institute, on the St Jérôme campus in Marseille in the Mosaic team, a network of 45 researchers (permanent, PhD students, postdocs) working at the interface between microscopy and biology.
The thesis will be supervised by Guillaume Baffou, 40, CNRS researcher (http://guillaume.baffou.com).
Constraints and risks
- use of lasers
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