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PhD Fellowship W/M

This offer is available in the following languages:
Français - Anglais

Date Limite Candidature : vendredi 8 juillet 2022

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General information

Reference : UMR7210-ALACHE-001
Workplace : PARIS 12
Date of publication : Friday, June 17, 2022
Scientific Responsible name : Alain Chédotal
Type of Contract : PhD Student contract / Thesis offer
Contract Period : 36 months
Start date of the thesis : 1 October 2022
Proportion of work : Full time
Remuneration : 2 135,00 € gross monthly

Description of the thesis topic

This project will use large-volume tissue clearing and state-of-the-art three-dimensional (3D) light-sheet imaging of human embryo specimens to describe the ontogeny and organisation of the sexually dimorphic human pelvic plexus (also known as the pelvic ganglia or inferior hypogastric plexus) and its connections with the spinal cord. The project design incorporates specific parallel studies in mice, to maximise the translatability of new knowledge.

The project will focus on the human pelvic plexus. These highly complex aggregations of neurons located in the pelvis are responsible for the autonomic regulation of urogenital organs and co-ordination of many of their critical functions. Specific parallel studies will be performed in mice. This project will use a vast library of pre-validated primary antibodies to reveal the neurotransmitter and transcriptional identities of pelvic ganglion neurons and describe their connectivity.

Work Context

Image Data will be analysed using innovative pipelines, including virtual reality to visualise and accurately segment data in 3D. It will apply both machine learning and deep learning models for unattended image analysis for unbiased quantitative data segmentation.
The aim of the project is to provide the first extensive visualization and analysis in two areas of the developing human nervous system: the lumbosacral spinal cord and the pelvic plexus (pelvic ganglia). Specific aspects of this neural circuit development will also be examined in mice. The outcomes will provide the first insights into the developmental processes underlying this complex neuronal network that uniquely merges autonomic and volitional motor systems. The projects will build the first 3D atlases of the neuronal organisation and axonal connectivity of the developing human pelvic plexus and lumbosacral spinal cord. These fundamental resources will benefit researchers, anatomists and clinicians. Selected parallel studies on developing mouse systems will provide new mechanistic insights that can be examined experimentally in the developing mouse system.
the project is part of a collaborative program between the CNRS and the University of Melbourne, involving a CNRS team (A Chédotal) and an Australian team (Janet Keast/Peregrine Osborne)

Constraints and risks

Use of microscopes with lasers
Work on fixed human and murine embryonic tissues
frequent work on computers and with virtual reality headsets
One year stay in Australia (Melbourne) as part of the collaboration and PhD thesis
A good knowledge of spoken and written English is essential

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