Doctorat M/F en microscopie & analyse d'image pour étudier le noyau 3D des plantes

Job Description

Thesis Subject

Objectives: Heat stress affects heterochromatin organization and nuclear movement, but the impact remains unknown at the gene scale. This IRP aims to quantify the mobility and the transcriptional activity depending on the relative positioning within the nucleus, depending on the chromosome positioning. Measure will be performed under control and heat stress conditions, in wild-type and in plants impaired in two mutants affected in temperature sensing and adaptation (hsfA2 and hsp101). (Obj1) Quantify the mobility of 12 chromatin regions labelled with the ANCHOR system80 during heat wave response (27°C, 37°C) using ChromTrack3D. (Obj2) Quantify the chromatin mobility of three candidate loci responding to heat stress (27°C, 37°C) in wild-type and in plants affected by the heat stress response using ANCHOR and ChromTrack3D. (Obj3) Quantify the expression changes of the three candidate loci with an allelic resolution using the MS2 tag and single molecule RNA FISH coupled with ANCHOR. In parallel, a collaboration with WP3 and NVT (S1) will be realized to optimize our existing gene tracking method81 for long-term (up to 12 h) live imaging enabling chromatin mobility measurements in 3D (ChromTrack3D). In addition, the use of microfluidic devices will be implemented to reduce the stress on plants generated by long-term experiments (S2).
Expected Results: R1. A ready-to-use protocol for long-term gene position tracking and analysis using the ANCHOR system directly in planta. R2. Time-lapse image datasets of ANCHOR-tagged loci during heat stress responses in wild-type and mutants. R3. Quantitative data on gene mobility and transcription levels relative to R2. R4. A working model of the spatio-temporal transcriptional reprogramming during the heat stress response in the nucleus.

Your Work Environment

This work will take place at iGReD, a research laboratory composed of 15 teams, within the Chromatin and 3D Nuclear Dynamics (CODED) team. The CODED team studies how various aspects of chromatin organization enable an appropriate transcriptional response during development or in response to environmental stimuli, using molecular, genetic, and bio-imaging approaches. The doctoral candidate will be part of a european MSCA-DN network named AGILE (www.msca-agile.eu) and will participate in all the traniing and events orgniaed within the frame of the AGILE project and described in the website. In addition, a direct collaboration with the NeoVirTech compagny (Toulouse, France) and with CEA (Cadarache, France) is part of the project.

Compensation and benefits

Compensation

2300 € gross monthly

Annual leave and RTT

44 jours

Remote Working practice and compensation

Pratique et indemnisation du TT

Transport

Prise en charge à 75% du coût et forfait mobilité durable jusqu’à 300€

About the offer

About the CNRS

The CNRS is a major player in fundamental research on a global scale. The CNRS is the only French organization active in all scientific fields. Its unique position as a multi-specialist allows it to bring together different disciplines to address the most important challenges of the contemporary world, in connection with the actors of change.

CNRS

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