Description du sujet de thèse

Study of the regulation of cell adhesion mediated by integrins
in synaptic plasticity.
The candidate will mainly use fluorescence microscopy (traditional and super-resolution) to study the nanoscopic organisation of biomolecules involved in cell adhesion, in particular integrins and their interaction partners during synaptic plasticity. The candidate will be required to present his/her research results (oral presentations, writing of reports and publications) and to monitor the literature on the subject. He/she will also be involved in the life of the team (orders, maintenance, stock management, etc.) and of the laboratory (logistics, contribution to experimental set-ups, internal communications, etc.).
Activities:
- Cell biology
Cell culture (immortalised lines, dissociated or sliced neurons (rat/mouse)
Transfections (electroporation, lipofection, etc.), Viral infections (Adenovirus, Lentivirus)
Biological chemistry (metabolic labelling, etc.)
Microscopy
Cell culture (immortalised lines, dissociated or sliced neurons (rat/mouse)
Fluorescence imaging (Epifluorescence, Confocal, dSTORM, DNA-PAINT, sptPALM, etc.)
Image analysis (Fiji, Imaris, Matlab, Picasso, PoCa, etc.)
-Laboratory life
Preparation and oral and/or written presentations of research results (team meetings, institute meetings, activity reports, publications, conferences, etc.).
Bibliographic monitoring.
Participation in collective tasks. (orders, logistics, etc.).

Contexte de travail

The IINS brings together 180 scientists of different nationalities and from different backgrounds (chemistry, structural biology, molecular and cellular biology, biophysics, clinical medicine, advanced imaging, neurobiology) to understand how the brain functions in healthy and pathological states. The IINS conducts multidisciplinary research at the cutting edge of synapse biology and the physiology of neuronal systems, with an emphasis on developing new technologies and tools.
Most of the work will be carried out on site (IINS/BIC, Centre Broca Nouvelle Aquitaine, Campus Carreire, Bordeaux) during the laboratory's opening hours (7am-9.30pm). Local travel cannot be ruled out, however, due to the collaborative nature of the project and depending on the experiments planned (in particular with the Institut des Sciences Moléculaires (ISM, Talence) and the Institut Européen de Chimie et Biologie (IECB, Pessac)). National and international travel is to be expected for training courses and conferences, depending on opportunities, the progress of the project and the scientific context.

Contraintes et risques

Possibility of working shifts