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PhD student in molecular and cellular biology of bacteria M/F

This offer is available in the following languages:
Français - Anglais

Date Limite Candidature : vendredi 26 août 2022

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General information

Reference : UMR5100-FRACOR-003
Workplace : TOULOUSE
Date of publication : Tuesday, August 16, 2022
Scientific Responsible name : Francois Cornet
Type of Contract : PhD Student contract / Thesis offer
Contract Period : 36 months
Start date of the thesis : 1 October 2022
Proportion of work : Full time
Remuneration : 2 135,00 € gross monthly

Description of the thesis topic

Dynamics of bacterial replicons during the cell cycle.
In bacteria, the steps of replication, segregation and cell division are not separated in time as in eukaryotes. These events are linked over time, and many actors participate in the choreography for the proper unfolding of these processes. In enterobacteria, the terminal region of the chromosome (ter) is the last to be replicated, separated and segregated. Its segregation is coupled to its subcellular position by the proteins MatP, which specifically recognises ter, and FtsK, which couples its separation to cytokinesis. On the other hand, intercatenation links between sister replicons and their resolution by topoisomerase IV (TopoIV) emerge as the main factors in the cohesive maintenance and subsequent controlled separation of newly replicated ter regions.
The project aims to understand how the action of TopoIV in the ter region is controlled to ensure precise spatiotemporal control of segregation and its coupling to cell division. We will use a synthetic biology approach by inserting known ter elements onto different types of plasmids. We will then characterise the behaviour and segregation of the replicons by video-microscopy (when and where in the cell) using fluorescent markers in living cells, as well as the decatenation by TopoIV using different molecular biology techniques.

Work Context

The Centre for Integrative Biology (CBI) is an institute managed by the CNRS and the University Paul Sabatier - Toulouse 3, located on the University campus in Toulouse. The CBI develops multidisciplinary and multi-scale approaches, from single molecules to whole organisms and animal societies, applied to many model organisms, from bacteria to humans. The CBI brings together about 400 people in its 38 research teams in three units: Microbiology (LMGM), Molecular, Cellular and Developmental Biology (MCD) and Animal Cognition (CRCA).
The Genome Dynamics (GeDy) team is part of LMGM and studies the maintenance functions of bacterial replicons, their integration and regulation with gene transfer and the cell cycle. Consisting of 13 permanent staff, including 9 researchers and teacher-researchers, and 5 PhD students or fixed-term contracts, our team studies a variety of biological models and techniques that allow us to understand molecular microbiology issues from various angles. The project proposed here will be co-supervised by Francois Cornet, DR-CNRS co-leader of the team, and Estelle Brendon, MCF UT3, and is part of a collaborative project with the teams of Olivier Espeli (CIRB, Paris) and Ivan Junier (TIMC, Grenoble), financed by an ANR contract.

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