PhD Position: Determining the Dynamic Three-Dimensional Environment of Telomeres (M/F)

Job Description

Thesis Subject

Exit from mitosis is a key stage in genome reorganization in mammalian cells.
At the beginning of G1 phase, the nuclear envelope reforms and chromosomes progressively regain their organization within the nucleus. However, the mechanisms allowing chromosomes to reach their final position remain poorly understood.
Telomeres, located at the ends of chromosomes, may actively contribute to this reorganization. Indeed, their position within the nucleus changes throughout the cell cycle, and they transiently interact with the nuclear envelope during its reformation. These observations suggest that telomeres may contribute to the dynamics of three-dimensional genome organization. However, their spatial environment and interactions with chromatin remain largely unknown. The repetitive nature of telomeres makes it difficult to study their interactions using conventional three-dimensional genome mapping approaches. The development of new approaches such as Micro-C and, more recently, Region Capture Micro-C (RCMC), provides an opportunity to investigate these interactions at higher resolution. The aim of this PhD project is to characterize the three-dimensional environment of telomeres throughout the cell cycle using the RCMC approach. The project will also investigate the molecular mechanisms underlying the interactions identified.

Your Work Environment

Research environment and supervision

The PhD student will join a research team specialized in molecular biology and genome organization, providing a stimulating and collaborative scientific environment within the Toulouse Integrative Biology Center (CBI Toulouse). The project will be supervised by Kerstin Bystricky and David Umlauf. Weekly lab meetings will be organized to closely monitor the progress of the project and discuss experimental results, allowing the research strategy to be adjusted when necessary. The PhD student will also present their work during team meetings, internal seminars and, where appropriate, at national and international conferences. A thesis committee will be established and will meet annually to assess the PhD student's progress, the quality of supervision, and the conditions under which the project is being conducted. The committee will provide recommendations to ensure the successful completion of the PhD project.

Constraints and risks

Risks associated with chemical products and reagents
Risks associated with biological agents
Risks associated with laboratory and work equipment
Risks associated with working alone
Risks associated with prolonged screen-based work

Compensation and benefits

Compensation

2300 € gross monthly

Annual leave and RTT

44 jours

Remote Working practice and compensation

Pratique et indemnisation du TT

Transport

Prise en charge à 75% du coût et forfait mobilité durable jusqu’à 300€

About the offer

About the CNRS

The CNRS is a major player in fundamental research on a global scale. The CNRS is the only French organization active in all scientific fields. Its unique position as a multi-specialist allows it to bring together different disciplines to address the most important challenges of the contemporary world, in connection with the actors of change.

CNRS

The research professions