Informations générales
Intitulé de l'offre : Research Engineer in Cellular and Molecular Biology applied to epidermal cells (M/F) (H/F)
Référence : UPR4301-LAELEG-054
Nombre de Postes : 1
Lieu de travail : ORLEANS
Date de publication : vendredi 24 octobre 2025
Type de contrat : IT en contrat CDD
Durée du contrat : 31 mois
Date d'embauche prévue : 1 janvier 2026
Quotité de travail : Complet
Rémunération : from € 2,496.98 gross monthly depending on experience
Niveau d'études souhaité : BAC+5
Expérience souhaitée : 1 à 4 années
BAP : A - Sciences du vivant, de la terre et de l'environnement
Emploi type : Ingenieure ou ingenieur en experimentation et instrumentation biologiques
Missions
As part of the CBM's NEUR2IT team, the successful candidate will be responsible for screening purified plant-based active ingredients and/or ingredients for their anti-ageing properties. The screening technology is based on a microRNA-inducible bioluminescent molecular probe called RILES (RNAi-Inducible Luciferase Expression System), which has proven effective in identifying bioactive compounds for dermatological (psoriasis) and anti-cancer (glioblastoma) applications.
Activités
The candidate will
- perform cell culture
- be responsible for biochemical activity tests (western blot, ELISA, colorimetric assay)
- be responsible for molecular activity tests (transfection, qRT-PCR, plasmid preparation).
- Propose strategies for screening bioactive compounds for various applications
Compétences
1) Proven experience in skin cell culture, particularly primary keratinocytes.
2) Proficiency in transfection techniques and, ideally, cell transduction.
3) Initial practical experience in genome editing using the CRISPR/Cas9 system would be appreciated.
4) Knowledge of skin objectification methods, particularly in the context of skin ageing, would be an asset.
5) Articles writing
6) Teamwork
Contexte de travail
The Centre for Molecular Biophysics (CBM) is a CNRS unit with approximately 150 staff members conducting research at the interface of chemistry, biology and physics, focusing on the molecular mechanisms of life and the dysfunctions that lead to the development of certain diseases. The person recruited for the InflammamiR project, funded by the APR-IR (Centre Val de Loire Region Project), will work within the NEUR2IT team, which is part of the BIOBRAT department. The successful candidate will collaborate with a renowned industrial partner in cosmetics and an academic partner with expertise in deep learning algorithms.
Working conditions:
Work location: laboratory located on the CNRS campus in Orléans La Source, set in 4 hectares of grounds.
Working hours: full-time - flexible hours.
Benefits: on-site catering, contribution to health insurance, sustainable mobility package, social activities (childcare, sports and leisure, ticket office, etc.).
Contraintes et risques
Common risks associated with experiments in biology and chemistry laboratories and in cell culture (L2)