Missions

The research engineer conducts experiments and analyzes results in order to obtain and study biological samples. In this project, we are studying red pulp macrophages (RPMs) in the spleen, which ensure the continuous phagocytosis of aged or damaged red blood cells. We are studying the adaptation of RPM metabolism to this function, particularly the contribution of autophagy, in a transgenic mouse model.

Activités

• Manage crossbreeding and stocks of transgenic mouse lines
• Carry out animal experimentation protocols
• Define the coherent set of biological techniques necessary for the experimental implementation of the research project
• Design the development and lead, as a specialist, the implementation of a project in a field of biology (sample preparation, flow cytometry, qPCR, confocal microscopy)
• Process data (analyze, interpret, and validate results)
• Record, format, and present results
• Disseminate and promote results and technological achievements
• Monitor scientific and technological developments
• Participate in the drafting of reports and funding applications
Related activities:
• Participate in the research team's common tasks
• Supervise students (Bachelor's to Master's level)

Compétences

• General knowledge of biology
• Genetics and/or genomics
• Ability to write technical procedures
• English level B1 according to the Common European Framework of Reference for Languages; B1 corresponds to an independent user level
• Level 1 animal experimentation training certificate (designer)
• Minimum degree required: Master in immunology

Contexte de travail

The person recruited will join the CNRS UPR3572/I2CT laboratory at the Institute of Molecular and Cellular Biology (IBMC) located on the central campus of the University of Strasbourg, France.
• A major player in fundamental research worldwide, the French National Center for Scientific Research (CNRS) is the only French organization active in all scientific fields. Its unique position as a multi-specialist allows it to combine different scientific disciplines to shed light on and understand the challenges of the contemporary world, in collaboration with public and socio-economic actors.• The CNRS UPR3572/I2CT “Immunology, Immunopathology and Therapeutic Chemistry” laboratory focuses on the molecular and cellular foundations of the immune response and its dysfunctions with the aim of defining new therapeutic avenues for targeted immuno-intervention in the treatment of autoimmune, tumor and viral diseases. It has state-of-the-art equipment in cytometry, microscopy, and transcriptomics. • The “Interactions of the Immune System with its Microenvironment” group studies the functional relevance of interactions between innate and adaptive immune cells and their immediate environment (mesenchymal cells, endothelial cells, epithelial cells, and neurons) in health and disease. The proposed work, led by V Flacher (CNRS Research Director), is based on collaboration with partners in the ANR AUTOMATE consortium (EL Gautier, ICAN, Paris; F Gros, CRBS, Strasbourg).

Contraintes et risques

Usual constraints associated with cell biology laboratory work.
Usual constraints associated with animal experimentation.
Work in a BSL2 confined laboratory.

Informations complémentaires

Working hours: 38.5 hours per week, Monday to Friday, daytime hours.
The Institute is located in the heart of the University of Strasbourg's central campus, accessible by bus and tram. The CNRS contributes to the cost of private health insurance and public transportation. An administrative restaurant is available on campus. Meals are covered by the CNRS.