Missions

The candidate will develop cutting-edge imaging methods, such as Single Molecule Localization Microscopy (SMLN), for the analysis of cell trafficking processes. He or she will participate in the preparation of cell cultures and transfections, using the molecular biology tools developed in the laboratory. L2 laboratory culture of HEK cells or primary culture of mouse neurons.

Activités

- Development of cell imaging protocols for tracking particles and vesicles using super-resolution microscopy.
- Development of cell lines by transient or stable transfection with fluorescent reporters.
- Development of new detection tools by cloning antibody fragments.
- Analysing, presenting and promoting the results obtained;
- Développement de nouveaux outils de détection par clonage de fragments d'anticorps.
- Analyser, présenter et valoriser les résultats obtenus ;

Compétences

General, theoretical or disciplinary knowledge:
- Expertise in super-resolution fluorescence microscopy and cell culture. - Mastery of bioinformatics tools for processing imaging data.
- In-depth knowledge of cell biology

Operational skills
- Mastery of sample preparation methods for super-resolution microscopy;
- Mastery of fluorescence microscopy acquisition techniques;
- Mastery of cell culture in the L2 laboratory for human cells (HEK) and primary cultures of mouse neurons;
- Mastery of cell transfection using plasmids or viral vectors;
- Experience in molecular biology would be an asset;
- Ability to communicate and ensure scientific exchanges with project collaborators; - Ability to work as part of a team and collaborate in a multi-disciplinary context;
- Ability to speak and write English (B2 level according to the Common European Framework of Reference for Languages) to present results at conferences and write publications.

Contexte de travail

The Laboratoire des Maladies Neurodégénératives is a joint research unit of CNRS, Université Paris-Saclay and CEA. Research focuses on neurodegenerative diseases such as Alzheimer's, Parkinson's and Huntington's.
The unit employs 60 scientists, grouped into 6 research teams.
The successful candidate will join the MIINDt team (Multimodal Integrative Neurodegenerative Diseases and Therapies). The team is currently relocating to a new research site (INSERM unit U1195 at Bicêtre hospital), where the research will take place.
The candidate will benefit from state-of-the-art equipment in the fields of super-resolution imaging and cell biology. The project is part of an ANR project, “HeparAlz”, conducted in collaboration with the “GLY-CRRET” team headed by Dr Papy-Garcia (UPEC, Créteil).

Contraintes et risques

Work in a research laboratory environment, using level 2 confined laboratories.
- Compliance with laboratory health and safety rules.