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Reference : UMR9199-ANSPER-001
Workplace : FONTENAY AUX ROSES
Date of publication : Monday, January 13, 2020
Type of Contract : FTC Scientist
Contract Period : 6 months
Expected date of employment : 1 April 2020
Proportion of work : Full time
Remuneration : from 2600€ gross monthly according to experience
Desired level of education : PhD
Experience required : 1 to 4 years
Postdoctoral Fellow (M/F), 6-month fixed-term contract to improve and use new human cellular models to understand the role of HTT and the impact of Huntington's disease (HD)mutation in striatal and cortical neurons in the brain of patient. Both types of neurons are the most affected in HD. Human neurons are differentiated from several wt, mutant and gene-edited human pluripotent stem cell lines (iPS).
Huntingtin (HTT) is a scaffold protein involved in many cellular functions essential to the survival and the activity of neurons. HD is a CAG trinucleotide repeat disorders caused by the length of a repeated section of exon 1 exceeding a normal range.
The project will combine a wide range of cell biology approaches with biochemical and imaging analyses. The candidate will be ask to :
- Produce different HTT-gene edited iPS clonesusing CRISPR-Cas9 technology.
- Amplify, QC, bank and differentiate into neural cells iPSC lines
- Analyze different cell phenotypes related to the modulation of HTT activity using biochemical or imaging technics on live or fixed cells
- Analyze and communicate the results produced
- Write scientific articles.
The candidate must have a PhD in neuroscience and a publication track record in science.
The candidate must be able to demonstrate his/her expertise in the culture and neuronal differentiation of human pluripotent stem cells and in genome editing using Crispr-Cas9 technology. He/she should be motivated by biomedical research, autonomous, rigorous and able to work in a team environment.
In addition, the candidate should have a strong background in neurobiology, cell biology and/or central nervous system biology.
The program is part of a long-standing effort to establish cellular models of neurodegenerative disorders using human pluripotent stem cells.
The present project focuses on the study of normal (i.e. non-mutant) HTT functions in a wt and pathological (HD) context. The applicant will join the team co-led by Dr. Anselme Perrier. This team focus on "Cell-Cell interactions in neurodegenerative diseases: Models and Biotherapies". The team is part of the CEA/CNRS/Univ Paris Saclay UMR9199 unit led by Dr. Emmanuel Brouillet in the MIRcen department led by Dr. Philippe Hantraye at the CEA centre in Fontenay-aux-Roses.
Constraints and risks
No specific risks other than those classically associated with cell biology.
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