Missions

As part of the ANR ENHANCE project, the Molecular Microbiology of Actinobacteria team (I2BC Microbiology Department) is recruiting a postdoctoral researcher to participate in the study of negamycin biosynthesis in Kitasatospora purpeofusca. The successful candidate will be responsible for characterizing the biosynthesis gene group.

Activités

- Cultivate bacterial strains (Streptomyces, Kitasatospora/Escherichia coli)
- Construct mutant strains (gene inactivation/deletion)
- Clone and express genes in Streptomyces/Kitasatospora/Escherichia coli
- Participate in the analysis of mutant strains (LC or LC-MS analysis of culture supernatants).

Compétences

- Strong command of microbiology and molecular biology techniques
- Ability to organize and conduct experiments independently
- Ability to work as part of a team
- Ability to get involved in the day-to-day running of the team
- Knowledge of handling actinomycetes would be appreciated
- Knowledge of analytical chemistry techniques (particularly HPLC and LC-MS) would be appreciated
- Good level of English

Translated with DeepL.com (free version)

Contexte de travail

The I2BC is a large joint research unit of the CNRS, CEA, and Paris Saclay University, created on January 1, 2015. Located on the Gif-sur Yvette and Saclay campuses, the unit has an average staff of approximately 650 people divided among five scientific departments, 60 research teams, and 17 high-level technology platforms.
The “Molecular Microbiology of Actinobacteria” team focuses on the biology of actinobacteria, and more specifically on the various aspects of their specialized metabolism.

Contraintes et risques

Possible travel to France or Germany to meet with project partners