Missions

The candidate will lead a project aimed at characterizing the interactions between the IpaA effector protein of Shigella and the host cell focal adhesion protein vinculin. The recruited candidate will build tools using molecular biology techniques, work on purified proteins systems and study interactions using High-Speed Atomic Force microscopy, as well as in vitro cultured epithelial cells infected by bacteria.

Activités

The candidate will have the following activities:

• Study pathogenic bacteria-host cell interactions in cell culture models.
• Characterize the effects of vinculin complexes on actin filaments using high-speed atomic force microscopy.
• Conduct, by adapting the experimental conditions, a set of techniques (electrophoresis, immunological, histological techniques, cloning, purification of recombinant proteins, cell culture, manipulation of class 2 pathogenic bacteria (Shigella), fluorescence microscopy.
• Exploit and present analysis results
• Writing experience or study reports
• Generate and organize technical resources as part of the project
• Ensure monitoring and transmission of results.
• Ensure scientific and technological monitoring in the field associated with the project
• Participate in the dissemination and promotion of results in the form of oral presentations and publications

Compétences

Knowledge
• Molecular Biology, High Speed Atomic Force microscopy, Biochemistry.
• Health and safety regulations
• Legal and ethical framework
• English language: B1 to B2 (common European framework of reference for languages)

Operational skills
• Protein purification techniques (ion exchange affinity chromatography)
• Cell biology techniques (cell culture, transfection)
• Knowledge of the research environment and scientific English
• Ability to interact with students and researchers

Expertise
• Cell culture techniques
• Immunofluorescence markings
• Mastery of protein manipulation techniques
• High Speed Atomic Force Microscopy

Soft skills
• Show rigor
• Have a sense of organization
• Know how to work in a team
• Know how to adapt

Contexte de travail

The main research theme of the I2BC laboratory concerns the characterization of the integrated functioning of the cell and in particular the understanding of the processes that govern the organization and overall physiology of the cell. Researchers carry out their work on different organisms (from viruses to human cells) or on complexes reconstituted in vitro, at different scales and under various physiological conditions. The Department of Cellular Biology within the I2BC includes 10 teams studying the mechanisms that regulate biogenesis, the organization and maintenance of specialized structures and compartments of the cell, intracellular trafficking and exchanges between compartments, the actin cytoskeleton in physiological and pathological conditions.

The CaSMI (Calcium signaling and Microbial Infections) team associated with the Department of Cellular Biology studies the interactions between pathogenic bacteria and host cell leading to the crossing of the intestinal barrier in the case of the enteroinvasive bacteria Shigella and the blood-brain barrier by the pneumococcus .

Contraintes et risques

• Experiences in an L2 environment
• Collaborative work involving joint meetings and travel between the I2BC in Gif-sur-Yvette, 91190 and the NanoLSI Institute, Kanazawa, Japan.