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Reference : UMR9198-PHIBOU-001
Workplace : ORSAY
Date of publication : Sunday, December 01, 2019
Type of Contract : FTC Scientist
Contract Period : 30 months
Expected date of employment : 1 March 2020
Proportion of work : Full time
Remuneration : between 32 346 to 46 096 € gross annual income according to experience.
Desired level of education : PhD
Experience required : Indifferent
- Study the function of regulatory RNAs from the opportunistic pathogen Staphylococcus aureus.
- Identify regulatory-RNA-associated phenotypes. In particular, identify regulatory RNAs contributing to antibiotic resistance.
- Improve an existing method to identify regulatory RNA targets.
- Establish networks associated with regulatory RNAs.
- Generate experimental data that will be processed by our bio-computing partner to model regulatory/target RNA interactions.
- "Ribosome profiling" experiments.
- RNA-Seq/DNA-seq data analysis (in collaboration with our partners).
- Molecular genetics (plasmid construction, gene fusion, site-directed mutagenesis, Northern blots, Western blots, enzymatic assays).
- Handle class 2 pathogen (Staphylococcus aureus) with mutant constructions.
- Handle radioelements (P32) for Northern blot experiments.
- Keep up to date with bibliography
We seek a candidate that is strongly motivated by the study of regulatory RNAs and the challenge associated with the project. He/she must like to work on team projects. He/she should have a broad scientific culture in biology and be autonomous to perform molecular genetic experiments. Additional expertise in ribosome profiling, microbiology and bioinformatics will be a support for the candidate recruitment.
- Molecular biology.
- Bacterial genetics.
- Bacterial physiology.
- Data analysis.
- Conservation and dissemination of results obtained.
The project is financed by an ANR 2020-2023 grant. It will be carried out at the Institute of Integrative Cell Biology (I2BC). At present we are located on the Orsay University campus. However, in 2020, we should move to a new building on the CNRS campus in Gif-sur-Yvette. The project will benefit from the resources made available by the I2BC (e.g. common facilities, secure P2 laboratories) and privileged access to the Institute's scientific platforms. The two project partners are I2BC teams.
Constraints and risks
Handle Staphylococcus aureus (class 2 pathogen)
Handle radioelements (P32)
Handle chemicals routinely used for microbiology and molecular biology.
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