Reference : UMR8214-VERSAR-002
Nombre de Postes : 1
Workplace : ORSAY
Date of publication : Thursday, January 19, 2023
Type of Contract : FTC Scientist
Contract Period : 10 months
Expected date of employment : 1 April 2023
Proportion of work : Full time
Remuneration : 2889,51 € (monthly gross salary)
Desired level of education : PhD
Experience required : 1 to 4 years
This 10 months post-doc is funded by the ITMO cancer, in the program Interdisciplinary approaches to oncogenic processes and therapeutic perspectives. The goal is to obtain the proof of concept that radioamplifying fluorescent metallic nanoparticles can be used as nanodosimeters in comparison with fluorescent molecules.
This work will be done in collaboration within iNanoTheRad, between laboratories of different and complementary disciplines and expertise:
- ISMO CNRS/Paris Saclay: the NanoBio team has expertise in the synthesis of grafted metallic nanoparticles, and the quantification and characterization of their efficiency in living cells when activated by medical beams (hadrontherapy in particular).
- LIDYL / DICO at CEA: the team has expertise in the measurement of fluorescence lifetimes of dyes, in particular rhodamines, by the TCSPC method; DICO is also recognized as a reference group in radiation chemistry and the use of ionizing radiation and femtosecond and picosecond pulsed lasers.
Metal nanoparticles (NPs) grafted to a fluorophore will be synthesized at ISMO using the group's patented method. The recruited researcher will be in charge of preparing cell samples containing the fluorescent NPs under classical conditions (2D) and also 3D models. A collaboration with the LIDYL (CEA) will allow the researcher to optimize a transportable time-correlated single photon counting system to measure the fluorescence lifetime of the NPs. The fluorescence lifetime measurement will be performed using a single photon counting system. The transportable system will then be moved to other teams' laboratories to collect measurements on 2D and 3D in vitro cell models.
Nanoparticle synthesis and characterization
Preparation of cellular samples
In view to improving and personalizing radiation therapy treatments, innovative strategies are rapidly developing. In particular multimodal nanoparticles (NPs) are used to improve the effects of radiations and as well as the performances of medical imaging. Interestingly, the same agents can be used to quantify the dose induced at nanoscale, a key issue to not only characterize the elementary mechanisms in cells but also quantify and predict their efficiency (nanodosimeter). The project aims to develop an experimental protocol to evaluate the dose deposition induced during a treatment with radiation and nanoparticles. In this perspective, nanoparticles grafted with a fluorescent molecule, will be used as nanodosimeters. It is known that the fluorescence lifetime provides information on the environment changesSo, this measurement in cells loaded with NPs, treated by radiation (medical x-rays and protons), will allow to evaluate the local dose and, in the end, the effectiveness of the combined NP-radiation protocol. The measurement of tau will be carried out with a multichannel TCSPC. The Mefisto project proposes to purchase and implement a transportable and adaptable TCSPC to perform dose measurements in several laboratories and conditions.
Constraints and risks
We don't expect any risk in term of scientific, technical, managerial and environmental risks.
We talk about it on Twitter!