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Portail > Offres > Offre UMR7654-ALEGAU-005 - Post-doc (H/F) biologie de la migration cellulaire

Post-doc (M/F) Biology of cell migration

This offer is available in the following languages:
Français - Anglais

Date Limite Candidature : samedi 20 août 2022

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General information

Reference : UMR7654-ALEGAU-005
Workplace : PALAISEAU
Date of publication : Saturday, July 30, 2022
Type of Contract : FTC Scientist
Contract Period : 6 months
Expected date of employment : 1 October 2022
Proportion of work : Full time
Remuneration : salary ranging from 2793 € to 3210 € net per month depending on experience
Desired level of education : PhD
Experience required : Indifferent

Missions

Characterisation of the WAVE shell complex, an alternative multiprotein complex to the WAVE regulatory complex in cell migration

Activities

- transfection of plasmids and siRNAs
- isolation of knock-out and knock-in cell lines by CRISPR/Cas9
- videomicroscopy
- Purification of multi-protein complexes by Tandem Affinity Purification
- writing research papers

Skills

- know how to grow mammalian cells
- know how to construct expression and genome editing plasmids
- know how to transfect and isolate stable clones
- know how to quantitatively analyse cell migration
- ability to speak English

Work Context

The Structural Biology of the Cell laboratory (CNRS UMR7654) at Ecole Polytechnique is composed of 4 teams representing a continuum of expertise ranging from computational biology to cell biology through structural biology. The post-doc will work in Alexis Gautreau's team, known in cell biology for its contributions to the study of the actin cytoskeleton.

Constraints and risks

Handling of cells classified as L2

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