Missions

The candidate will be integrated into the “Mechanism of meiosis” team at the Jacques Monod Institute. He/she will have the mission of developing molecular, cellular biology and mass spectrometry techniques in mouse and Xenopus oocytes in order to enable the advancement of the team's research themes.

Activités

-- Construction of molecular biology tools.
- Harvest and culture of mouse oocytes, videomicroscopy.
- Collection and culture of Xenopus oocytes, preparation of extracts, western blot.
- Analysis of mouse oocytes after immunostaining by confocal and super-resolution microscopy. Mounting and quantification of acquisitions.
- Development of new techniques for preparing protein samples for analysis by mass spectrometry.
- Being proactive in contributing to the smooth functioning of the lab

Compétences

- Prior experience working in a laboratory (internships or CDD) is essential.
- Knowledge of classic molecular biology techniques (PCR, cloning, RNA preparation), and cellular biology (culture, microscopy).
- Knowledge in specific techniques (harvesting and culturing mouse oocytes, evaluating meiotic maturation in mouse oocytes, preparing embryo culture media, knowledge of CRISPR-Cas9, prior experience with “RNA-FISH” technique)
- Knowing how to communicate in English is essential. No knowledge of French is required.
- Proactivity, rigor, perseverance, reliability and good ability to work in a team.

Résultats attendus et contrôles

The candidate is expected to contribute to a research project on Cyclin B3 and its role during meiosis and mitosis in direct interaction with an Assistant Professor and a CNRS Research director. They will oversee the experiments and conduct one-to-one meetings on a daily basis to ensure that experiments are conducted and reported correctly.

Contexte de travail

The host team is interested in the mechanisms of cell division in meiosis, particularly in mouse and Xenopus oocytes. It is made up of 3 doctoral students, 1 assistant engineer, 3 researchers, one assistant professor, one postdoc and 2 Engineers. Bachelor and master students are hosted all year round.
The activity is carried out at the Jacques Monod Institute, which is a joint CNRS - Paris Diderot University research unit, of around 250 people and 23 nationalities. The IJM is housed in a recent University building on the Paris Rive Gauche campus located in the 13th arrondissement of Paris and accessible by all means of transport (Metro 14, RER C, Tramway T3, bus 62 and 89.. .).

Le poste se situe dans un secteur relevant de la protection du potentiel scientifique et technique (PPST), et nécessite donc, conformément à la réglementation, que votre arrivée soit autorisée par l'autorité compétente du MESR.

Contraintes et risques

None