Missions

The post-doctoral researcher (initial contract of 3 months, renewable for up to 1 year) will be responsible for developing, conducting and analyzing experiments as part of research projects related to acute and chronic pain. The main objective of the project is to study the potential role of certain ion channels in the development and transmission of pain messages. The laboratory has various knockout and knock-in mouse models for certain genes encoding potassium channels of the two-pore domain channel family. One of the postdoctoral researcher's tasks will be to carry out sensitivity tests on these mouse lines, for example to mechanical or thermal stimuli. Other tests will also be carried out to assess other sensory and cognitive functions. Another task will be to carry out primary neuron cultures of these different animal lines to perform electrophysiological recordings. Different pain models will be carried out after injections of inflammatory substances and spontaneous and evoked tests will be performed. Finally, another task will be to perform histological sections of mouse nerve tissue for immunological or RNAscope labeling. All of this work is part of a pre-clinical approach to identifying new therapeutic targets for the treatment of pain.

Activités

- Electrophysiological recordings (patch clamp and TEVC)
- Behavioral tests on mice to quantify cognitive functions, motor skills and pain.
- Performing procedures on mice such as surgery, injections, sampling, marking, etc. - Formatting results and statistical analysis.
- Monitoring the literature and improving/adapting techniques and analyses according to research projects.
- Writing scientific articles.

Compétences

- Cell culture and microscopy - Molecular biology and cloning
- Electrophysiology on Xenopus cells or oocytes - Mouse surgery.
- Understanding and knowing how to implement behavioral tests in mice.
- Knowing how to analyze data: statistical analysis, electrophysiological signal analysis.
- Histology, immunohistochemistry.
- Training in animal experimentation and surgery.
- Organizational skills, rigour and method, motivation, autonomy, team spirit.
- Written and spoken English.

Contexte de travail

This position is available at the Institute of Molecular and Cellular Pharmacology in Valbonne - Sophia Antipolis (Alpes-Maritimes). The IPMC is a joint research unit (UMR 7275) formed by the CNRS, Inserm and the Université Côte d'Azur (250 people, 8000 m2 of buildings). Its 20 research teams benefit from a high-level technological environment in integrative biology, electrophysiology, molecular and cellular biology, imaging, cytometry, biomolecule analysis and functional genomics. In particular, the shared technical platforms for animal experimentation and the team's own equipment enable detailed phenotyping of mice. The postdoc will work in the “Physiology and Physiopathology of Potassium Channels” team under the direction of Delphine Bichet and Franck Chatelain.

Le poste se situe dans un secteur relevant de la protection du potentiel scientifique et technique (PPST), et nécessite donc, conformément à la réglementation, que votre arrivée soit autorisée par l'autorité compétente du MESR.

Contraintes et risques

Work on GMOs and animal models