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Reference : UMR7249-JERWEN-003
Workplace : MARSEILLE 13
Date of publication : Friday, November 12, 2021
Type of Contract : FTC Scientist
Contract Period : 12 months
Expected date of employment : 1 March 2022
Proportion of work : Full time
Remuneration : Between 2743 and 4072 € gross monthly salary depending on experience
Desired level of education : PhD
Experience required : Indifferent
The successful candidate will be part of the ERC Consolidator Grant “TryptoBoost” project carried out at the Fresnel Institute under the direction of Jérome Wenger. Our group has acquired a broad expertise in the nanoscale control of light fields in plasmonic nanostructures and its application to improve fluorescence spectroscopy applications by focusing on single molecules.
Our next project aims to efficiently monitor single proteins using their fluorescence enhanced by optical nanoantennas. Plasmonic optical nanoantennas enable analysis of a single protein at physiologically relevant micromolar concentrations through localization and enhancement of light-matter interactions at the nanoscale.
Ensuring that the target protein is located in the most appropriate position relative to the nanoantenna is the key to getting the most out of the enhanced fluorescence phenomenon. To this end, we wish to implement nano-optical trapping to precisely locate the protein target in the antenna hot spot for long investigation times.
Recently, it has been shown that nano-optic trapping traps a single protein in the space of a double nanhole antenna. However, the transmission measurements used in this earlier work were limited to very low contrast in the signal change induced by the presence of the protein. In contrast, our unique combination with time-resolved fluorescence will allow a much better signal without background.
Our detection will take advantage of our time-resolved fluorescence spectroscopy techniques and advanced nanonantenna designs, which are unique in this field. We aim to develop under the microscope a configuration combining an infrared laser to perform trapping with a visible laser to monitor fluorescence. The combination with time-resolved techniques will make it possible to quantify various parameters (trapping force, position and temperature of the trapped object, etc.) with unprecedented spatio-temporal resolutions.
- sample preparation
- conducting optical microscopy experiments
- results analysis
- writing of reports and articles
In order to strengthen our multidisciplinary team, we are looking for a physicist with expertise in experimental nanophotonics, plasmonics and / or optical trapping. To apply for the postdoctoral contract, applicants must hold an internationally recognized doctorate in physics or engineering.
Experience in a scientific project involving either nanophotonics, optical microscopy, single molecule fluorescence techniques and / or optical trapping will be highly appreciated.
The Fresnel Institute is a state research laboratory based in Marseille / France, dedicated to research and higher education. The Fresnel Institute seeks to recruit talented, enthusiastic and highly motivated young scientists to boost their careers as researchers in nanophotonics and associated technologies.
The position is in the form of a full-time assignment (38h / week, 12 months / year) under a CNRS contract. Contracts are offered for one year. The extension will be discussed based on performance.
Constraints and risks
Mastering laser-related risks
Selection is based on merit and potential, measured against record and personal achievement. Proactivity, participation in community activities and the ability to work as a team are also taken into account.
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