Missions

The aim of this project is to overcome the limitations of in vivo brain imaging by developing a flexible and robust multicore fiber-based coherent Raman lensless endoscope that will allow to retrieve chemical and structural information in a complete label-free manner. Hyperspectral CARS measurements will allow to efficiently visualize myelin in rodants, as well as identify and track several biomolecules through their vibrational spectrum. The developed multiphoton endoscope will also be compatible with other complementary multiphoton imaging techniques, such as sum-frequency generation (SFG) and two-photon excited fluorescence.
Reporter molecules such as GCaMP – a protein whose fluorescence depends on the concentration of calcium ions – allow to track the neuronal activity in vivo in mice, mimicking electrophysiology experiments. The flexibility, long-term stability and multi-modal imaging modalities of the developed multicore endoscope will allow to perform prolonged studies on free-roaming animals. In particular, the developed technology will be used to study the effect of myelin degradation and test the efficiency of drug therapies in live animals, enhancing our understanding of multiple sclerosis and other demyelination-related diseases.

Activités

The aim of the project is to develop a Coherent Raman (CARS) lensless endoscope to study demyelination related diseases, in particular multiple sclerosis, through in vivo deep brain imaging. The objectives are:
• Objective 1: Development of an optimal multicore fiber for multiphoton endoscopic imaging
• Objective 2: Development of an imaging CARS endoscope platform.
• Objective 3: Label-free study of demyelination and remyelination treatment in live mice.

Compétences

- Optical fiber
- Optical instrumentation
- Interfacing opto-electronic devices
- Data acquisition
- Data analysis
- Data presentation
- Modelling

Analyse de données
Présentation de données
Modélisation

Contexte de travail

The work will take place in a fully equipped optics lab.

Le poste se situe dans un secteur relevant de la protection du potentiel scientifique et technique (PPST), et nécessite donc, conformément à la réglementation, que votre arrivée soit autorisée par l'autorité compétente du MESR.

Contraintes et risques

Laser risk