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Reference : UMR7057-CARPHI-010
Workplace : PARIS 06
Date of publication : Tuesday, November 10, 2020
Type of Contract : FTC Technical / Administrative
Contract Period : 12 months
Expected date of employment : 1 January 2021
Proportion of work : Full time
Remuneration : CNRS gross salary between 2510 and 2890 euros depending on experience
Desired level of education : Higher than 5-year university degree
Experience required : Over 10 years
Extracellular vesicles (EVs), especially mesenchymal stem cells (MSCs), play a direct role in restoring tissue function and homeostasis. These EVs are increasingly seen as competent effectors as an alternative to cells for regenerative medicine in a paradigm shift from cell therapy to sub-cellular therapy (EV). The implementation of the protocol for the production of EVs in bioreactors compatible with Good Manufacturing Practices (GMP) is a major challenge in the implementation of EV MSC production.
We intend to set up a culture of MSCs in stirred tank GMP bioreactors to produce EVs. In addition, we will develop a formulation based on the resulting vesicles combined with a hydrogel and test their immunomodulatory effect in vitro and in vivo.
Required skills :
- production of adherent cells in agitated tank type bioreactors,
- production of extracellular vesicles and their characterization,
- in vitro and in vivo immuno-modulation tests
The person recruited will benefit from an internationally renowned interdisciplinary environment at the MSC laboratory, renowned for its expertise in the field of biophysics.
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