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Reference : UMR7025-BERBIH-001
Workplace : COMPIEGNE
Date of publication : Wednesday, October 13, 2021
Type of Contract : FTC Scientist
Contract Period : 3 months
Expected date of employment : 15 November 2021
Proportion of work : Full time
Remuneration : From 2 600 € / month
Desired level of education : Higher than 5-year university degree
Experience required : Indifferent
This project aims to develop a predictive test for recurrence or resistance of chronic myeloid leukemia based on the principle of aptasensor (contraction of 'aptamer' and 'sensor') recognizing STAT5 protein. This test will be based on the principle of FRET (Fluorescence Resonance Energy Transfer) where the aptamer is coupled to both a donor and a recipient (chromophore). The mission concerns the validation of each of the stages leading to a functional test.
A FRET system will be designed by identifying the different elements. Different systems have been tested: the first step of binding the aptamer to a donor (Quantum Dot) is validated, but the acceptor must be defined. This first step will be carried out with a model aptamer system (thrombin / anti-thrombin), the structural characteristics of which are known.
The second step will consist in the validation of a complete FRET system with a patented anti STAT5 aptamer, first in the presence of recombinant STAT5, then extracted from cells in culture, and finally, if possible, with relevant biological samples.
Practical skills in molecule characterization techniques (proteins, nucleic acids), knowledge in biophysics (fluorescence, methods for evaluating interactions) and cell biology (monitoring animal cell cultures, protein extraction) are expected. The candidate must demonstrate initiatives based on his/her scientific skills, rigor, in respect of scientific integrity (keeping a mandatory laboratory notebook).
Oral and editorial communication skills are also necessary, in respect of deadlines, as well as the ability to work in a team
The recruited research engineer will develop the project at the Enzyme and Cell Engineering laboratory, UMR CNRS 7025, at the University of Technology of Compiègne, in Compiègne (Oise). In particular, the work is part of the theme 'Biomimicry and Molecular diversity'. The project is funded through a Déclic support from CNRS Innovation.
Constraints and risks
The protein purification / extraction part will be carried out in a Level 2 safety laboratory, Biophysical, fluorescence and interaction measurements experiments will be carried out in a bioanalysis platform.
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