Reference : UMR6554-ELOROB-001
Nombre de Postes : 1
Workplace : NANTES
Date of publication : Monday, January 16, 2023
Type of Contract : FTC Scientist
Contract Period : 17 months
Expected date of employment : 1 April 2023
Proportion of work : Full time
Remuneration : between 2805 to 3963 € euros/month depending on the experience of the candidate.
Desired level of education : PhD
Experience required : Indifferent
The PostDoc will work in the framework of MAMIWATA research project (ANR JCJC) led by Dr. Elodie Robert. This project aims at using both satellite and on-field environmental data to assess health risks due to pathogenic bacteria, and especially the population dynamics of Escherichia coli (E. coli) in Burkina Faso and Niger surface waters. The effect of environmental factors (hydrometeorology) and the relevance of E. coli as fecal indicators for predicting transmission risks of diarrheal diseases will also be investigated. Diarrheal diseases are a leading cause of death in sub-Saharan Africa due to the use of surface waters for domestic purposes.
The PostDoc will contribute to three main missions of the project:
- Description of the spatial and temporal dynamics of pathogens and its relationship with E. coli free-living and particle-attached fractions of the water column.
- Significance of aquatic bacterial diversity to mitigate pathogen transmission.
- Identification of underlying reasons behind the partition between free-living and particle-attached E. coli.
The PostDoc will study a series of samples collected in the field during two years at the four study sites (Kapore and Tanvi in Burkina Faso and Bangou-Kirey and Kongou in Niger). He/She will have 3 main tasks:
- He/She will carry out the determination of pathogenic microorganisms and associated bacterial communities using 16S-rDNA sequencing methods and LAMP (Loop-mediated isothermal amplification) on both free-living and particle-attached bacterial fractions. A two-week-stay in Prof. B. Etchebarne's laboratory at MSU (Michigan State University, East Lansing, USA) is planned to be trained on the LAMP method.
- The PostDoc will also measure the particle size distributions of collected samples and will identify the nature of colloidal particles using Scanning Electron microscopy combined with Energy-Dispersive X-ray Spectroscopy. He/She will investigate the type of association reached between colloidal particles and bacteria using ultrathin sections of resin-embedded samples.
- The PostDoc will also participate in managing and analyzing field data (Suspended Particulate Matter, Dissolved Organic Carbon, E. coli MNP) and in the writing of publications stemming from MAMIWATA research project.
The PostDoc should hold a PhD in microbiology and be acquainted with electronic microscopy. Fluency in spoken and written English (minimum B2 level required), and the ability to work, communicate and interact in an international collaborative consortium, will be appreciated. The ability to handle large datasets and the knowledge of a programming language (e.g. R, Python), is essential. Finally, experience in molecular biology (PCR, qPCR, see sequencing library preparation) would be an asset.
The PostDoc will be placed under the joint-supervision of Dr. Elodie Robert (project coordinator) at LETG-Nantes (Laboratoire Littoral, Environnement, Télédétection et Géomatique), Thomas Pommier at LEM-Lyon (Laboratoire d'Ecologie Microbienne), and Bruno Lartiges at GET-Toulouse (Géosciences Environnement Toulouse). Assigned to LETG Nantes, the post-doctoral student is expected to spend an 8-month mission at LEM (Lyon) and 8-month mission at GET (Toulouse), in addition to the mission in the United States.
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