Missions

This project involves implementing magnetic transport of target molecules through different fluidic media. The aim is to purify DNA present in a biological sample within a single passive microfluidic chip in a single step, without fluidic actuation. Our innovative superparamagnetic nanoparticle (SPN) transport technology exploits integrated micromagnetic substrates. An exploratory device has already been built, comprising two fluidic chambers, the first containing the biological sample containing the DNA to be purified, the second containing the elution and analysis buffer. Design, development and experimental testing of innovative microfluidic devices. Evaluation of their integration into existing detection instrumentation. Supervision of research masters and 1st year PhD trainees. Weekly project progress meetings with LMGP and Magia Diagnostics. Drafting of project progress reports.

Activités

Development of current fluidic micro-cartridges: sizing of microchannels and substrates, laser cutting, assembly of miniature integrated magnetic components. Characterisation of purification: implementation of magnetophoresis (control of external magnetic sources), visualisation of magnetophoretic transport by fluorescence microscopy, evaluation of the transfer rate of biological molecules by qPCR. Integration into the diagnostic system: assessment of the efficiency of the overall system, proposed modifications to the current portable reader.

Compétences

Proficiency in the usual biology laboratory techniques: pipetting, mixing, dosing, PCR, epifluorescence microscope, etc. Experimental manual skills: micro-fluidics, prototyping, setting up manipulations on the bench, oscilloscope, etc. Ability to work in a team and manage cooperation between several groups (2 labs and 1 start-up) Writing skills (technical reports, scientific publications)

Contexte de travail

The Magnetic Microsystems research group is part of the G2elab Grenoble Electrical Engineering Laboratory, a joint research unit (UMR 5269) of Grenoble INP - UGA, Grenoble Alpes University and the CNRS. It covers a scientific spectrum ranging from the integration of magnetic materials to the design of microfluidic systems implemented in medical devices. Our group works in close partnership with the IMBM (Interface Matériaux - Matière Biologique) team at the LMGP (Matériaux et Génie des Procédés) laboratory in Grenoble.

Le poste se situe dans un secteur relevant de la protection du potentiel scientifique et technique (PPST), et nécessite donc, conformément à la réglementation, que votre arrivée soit autorisée par l'autorité compétente du MESR.

Contraintes et risques

The person recruited will work mainly in the LMGP laboratory (Phelma site), and will be required to carry out certain tasks in the G2Elab laboratory (FabLab, GreenER-ENSE3 site), and experiments on the biological platforms of CIME-Nanotech (Minatec site). You will also be expected to attend weekly meetings at the Magia Diagnostics start-up premises (25 Av Maréchal Leclerc, Echirolles). Working hours will be within the laboratories' official working hours.