Missions

The postdoctoral researcher will work on developing multiplex immunohistochemistry approaches using a newly acquired instrument in Bordeaux, starting from samples from glioblastoma patients as well as patient-derived tumors, in order to characterize immune subpopulations. The postdoctoral researcher will also use in vitro approaches to culture patient-derived cells with immune cells such as macrophages, and will also be involved in carrying out or assisting with in vivo experiments. This work will be conducted as part of a competitive grant obtained by a consortium bringing together French teams with complementary models, tools, and expertise in neuro-oncology, including cancer cell and immune cell metabolism, chemistry, and computational biology.

Activités

Main activities
• Implementation of multiplex immunohistochemistry techniques on patient samples as well as patient-derived tumors (xenografts).
• Conducting experimental assays required for the project (genetic and pharmacological manipulation of human cancer cells, culture of murine macrophages, cell biology experiments, biochemistry, metabolic profiling, measurement of metabolic activities, and in vitro imaging using confocal microscopy).
• Monitoring relevant scientific literature related to the project.
• Presentation of results during weekly laboratory meetings as well as at national and international scientific meetings.
Secondary activities
• Interaction with other team members and collaborators.
• Participation in the in silico modeling of metabolic regulations.
• Participation in in vivo experiments (access to immunodeficient animals and neurosurgery).
• Contribution to collective laboratory activities (e.g., meetings, seminars).

Compétences

Core competencies:
• Expertise in immunohistochemistry, cell and molecular biology, and metabolic analyses.
• Knowledge in neuro-oncology.
• Additional knowledge of brain metabolic systems or glioblastoma will be considered an asset.
• Language skills: proficiency in spoken and written English is essential within the international group.
Skills:
• Strong proficiency in human cell culture techniques, microscopy, and solid knowledge of metabolic fluxes, metabolomic and transcriptomic analyses.
• Ability to quickly adapt to new methodologies.
• Proficiency in using computer tools and software dedicated to data processing.
Personal qualities:
• The postdoctoral researcher must be motivated, rigorous, well-organized, and able to work independently.
Additional training opportunities aimed at enriching the postdoctoral researcher's knowledge may be offered during the fixed-term contract.

Contexte de travail

This position is part of the INCA PCSI FAMTOM project (Unraveling the function of tumor-associated macrophages expressing ML-IAP in glioblastoma), which brings together experts in neuro-oncology, chemistry, metabolism, and bioinformatics to investigate the functional role of tumor-associated macrophages (TAMs) expressing ML-IAP in glioblastoma. The project is embedded within the activities of FAMTOM, ensuring a stimulating scientific environment at the interface of cancer biology, immunology, and bioinformatics. The postdoctoral researcher will join the Glioblastoma Metabolism team at IBGC (CNRS, Bordeaux), led by Dr. Thomas Daubon, and will work in close collaboration with consortium partners including the teams of Dr. Macha Nikolski (Bordeaux), Dr. Olga Iranzo (Marseille), and Dr. Aurélie Tchoghandjian (Marseille).