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Portail > Offres > Offre FR550-BRUMIR-005 - Post-doctorat "Élucidation du rôle de la Ded1 de la levure, une ARN hélicase à boîte DEAD, dans l'élongation de la traduction" (H/F)

Post-doc "Elucidating the role of yeast Ded1, a DEAD-box RNA helicase, in translation elongation" (M/F)

This offer is available in the following languages:
Français - Anglais

Date Limite Candidature : vendredi 29 octobre 2021

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General information

Reference : FR550-BRUMIR-005
Workplace : PARIS 05
Date of publication : Friday, September 17, 2021
Type of Contract : FTC Scientist
Contract Period : 12 months
Expected date of employment : 1 January 2022
Proportion of work : Full time
Remuneration : Gross salary will vary with candidate's experience starting at 3106€/month before taxes (around 2500€ after taxes) for candidate with less than 2 years post-doctoral experience. See the official CNRS salary table for researcher at http://labexdynamo.ibpc.fr/fp-dynamo-paris/
Desired level of education : PhD
Experience required : Indifferent

Missions

Attention: You must not have resided or carried out your main activity (e.g. work or studies) in France for more than 12 months in the 3 years prior to the recruitment date.
You have research experience in the field relevant to the call.


Ded1 (DDX3) is a yeast DEAD-box RNA helicase that is functionally conserved from yeast to humans. It is an ATP-dependent RNA binding protein and an RNA-dependent ATPase. It can dissociate short RNA helices and remodel ribonucleoprotein complexes. However, it can also function as an RNA clamp that can be turned on or off by regulating the ATPase activity. We have found that Ded1 is negatively regulated by proteins in the signal recognition particle (SRP), and that it may be important in pausing ribosomes during translation of polypeptides into the endoplasmic reticulum (https://doi.org/10.1101/2020.11.08.373522). Moreover, Ded1 appears to have a secondary RNA binding site that is independent of ATP and specific for certain RNAs; this is reminiscent of the E. coli, protein DEAD-box DbpA that has a carboxyl-terminal domain that specifically binds helix92 of the ribosomal RNA. We are interested in characterizing this binding site and the RNA features that are recognized with the ultimate goal of better understanding the biological role of Ded1.
Identify and characterize the RNA substrates of the DEAD-box RNA helicase Ded1 and elucidate the biological role(s) of Ded1.
PI: Kyle Tanner, UMR 8261, tanner@ibpc.fr

Linder P, Jankowsky E. From unwinding to clamping - the DEAD box RNA helicase family. Nat Rev Mol Cell Biol. 2011 Jul 22;12(8):505-16. doi: 10.1038/nrm3154.
Hilal Yeter-Alat, Naïma Belgareh-Touzé, Emmeline Huvelle, Molka Mokdadi, Josette Banroques, N. Kyle Tanner. The DEAD-box RNA helicase Ded1 from yeast is associated with the signal recognition particle (SRP), and its enzymatic activity is regulated by SRP21. 2020 BioRXiv doi: https://doi.org/10.1101/2020.11.08.373522

Activities

- T7 RNA polymerase transcription of RNA substrates
- Use of electro-mobility-shift-assays (EMSA) for protein-RNA interactions
- Enzymatic assays of protein activity, specifically RNA-dependent ATPase activities
- Purification of recombinant proteins expressed in E. coli

Skills

Skills/knowledge
- General knowledge of molecular biology (cloning, transformation of E. coli, etc)
- General understanding of biochemistry (enzymology, purification of recombinant protein, etc)
- Preference for experience working with yeast
- General understanding of scientific English

Know-how
- work with RNAs and protein
- purification of recombinant proteins
- use of electro-mobility-shift-assays (EMSA)
- enzymatic assays (ATPase, etc)

Soft-skills
- Plan and put into effect a scientific study
- Organize daily work
- Capable of working within a group
- Maintain a laboratory notebook

Work Context

Experimentation will be done in CNRS UMR8261 in the Institut de Biologie Physico-Chimique on the Curie campus in the 5th arrondisement. All the material necessary is available in the laboratory or immediate vicinity.
The project is part of the COFUND FP-DYNAMO-PARIS programme, which offers a unique interdisciplinary environment and a fundamental research initiative in the centre of Paris to train the scientists of tomorrow in the field of physico-chemical biology. The goal of the research programme is to integrate knowledge on gene expression, structural and membrane biology, and bioenergetics in bacteria, chloroplasts and mitochondria in the general context of improving our understanding the biogenesis of energy-transducing membranes. These studies will involve cutting-edge technologies including structural biology (NMR, X-ray crystallography and CryoEM), RNA sequencing, mass-spectrometry, synthetic biology, microfluidics, computational modelling and visualization. Post-doctoral fellows will have the opportunity of developing their research projects in a dynamic scientific environment covering three main research themes: i) RNA biology from bacteria to chloroplasts, ii) Membrane system dynamics and iii) computational modelling of molecular assemblies.
Post-doctoral Associates will benefit from the supervision of internationally-recognised experts in their fields, a world-wide network of renowned institutions, cutting-edge research infrastructure and state-of-the-art technical know-how that will allow them to hone their scientific skills, make themselves known and increase their competitiveness on the labour market. Specific training will be proposed to ERs through attendance of the EMBO course on “Laboratory Leadership for Postdocs” included in a retreat in Padua, and involvement in dedicated complementary soft skill training events.

Constraints and risks

Fellowship period should start between January 1 and May 31 2022.

Additional Information

The application should be written in English and uploaded at https://emploi.cnrs.fr/. It should include a detailed CV, a minimum of two letters of reference, a letter of motivation describing your short and long term career objectives, and a signed declaration stating that you are in compliance with the mobility rule which stipulates that you have not resided or carried out your main activity (work or studies) in France for more than 12 months during the three years preceding the date of recruitment. In addition, the complete file must also be sent to Bruno MIROUX & Magdalena TORTYNA, respectively director and manager of the project at the following email address DYNAMOcofund@ibpc.fr (Tel: 33 1 58 41 50 64). See details on the website http://labexdynamo.ibpc.fr/fp-dynamo-paris/

The position is intended as an initial step in a research career, and the assessment of the applicants will primarily be based on their research qualifications and their potential as researchers with skills that complement and strengthen on-going research within the partner institutions.

Step 1. The applications of each eligible candidate will be evaluated by independent international experts within the relevant research theme. Eligible applicants will be evaluated based on merits, motivation for the program, academic excellence and personal qualifications such as collaborative and communication skills.

Step 2. A maximum of three candidates will be retained for a video interview with the independent experts for each open position (Nov 2021). After the interview, candidates on the short list will be invited to a conference call with the project supervisor and a DYNAMO board member to define the general scope of the project, the training and the research environment. Candidates will be asked to draft a 3-page project proposal, bringing their own original ideas and will be given 2 weeks to submit it.

Step 3. Each project proposal will be evaluated by one of the independent experts and the DYNAMO board member who evaluated the candidate in the previous steps. The final selection will be made based on the scores obtained at steps 1, 2 and 3. The minimum score must be above 20/40. Top candidate will be offered the postdoc position with a starting date from January 1- May 31, 2022.

The CNRS (Centre National de la Recherche Scientifique) is an equal opportunity employer. Furthermore, FP-DYNAMO-PARIS is committed to promoting the role of women in science and thus explicitly encourages women to apply.

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