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Portail > Offres > Offre FR550-BRUMIR-004 - Post-doctorat "Biologie structurale de complexes ARN-protéine" (H/F)

Structural biology of RNA-protein complexes (M/F)

This offer is available in the following languages:
Français - Anglais

Date Limite Candidature : vendredi 29 octobre 2021

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General information

Reference : FR550-BRUMIR-004
Workplace : PARIS 05
Date of publication : Friday, September 17, 2021
Type of Contract : FTC Scientist
Contract Period : 24 months
Expected date of employment : 1 January 2022
Proportion of work : Full time
Remuneration : Gross salary will vary with candidate's experience starting at 3106€/month before taxes (around 2500€ after taxes) for candidate with less than 2 years post-doctoral experience. See the official CNRS salary table for researcher at http://labexdynamo.ibpc.fr/fp-dynamo-paris/
Desired level of education : PhD
Experience required : Indifferent

Missions

Attention: You must not have resided or carried out your main activity (e.g. work or studies) in France for more than 12 months in the 3 years prior to the recruitment date.
You have research experience in the field relevant to the call.

Solve the structure and understand the mechanisms of complexes involved in RNA maturation
The research of the team “RNA biogenesis, architecture and interactions” led by C. Tisné is focused on structural biology of RNA-protein complexes involved in RNA maturation. RNA is heavily co- or post-transcriptionally modified, with the largest diversity of modifications found in tRNAs. The recent discovery of modifications in mRNAs and their involvement in the regulation of gene expression has fueled a new field of investigation in this oldest area of RNA research, which has so far focused mainly on tRNAs and rRNAs. Given the widespread presence of post-transcriptional RNA modifications in all classes of RNAs and in all areas of life and their dynamics, it is clear that RNA processing pathways and RNA functions must have evolved under the influence of RNA modifications. The interplay between RNA processing and RNA modifications has received little attention so far. We want to understand how RNA modifications and processing together determine RNA maturation and, in turn, control or influence RNA functions. Applicants for this position should propose a project using structural biology to address these questions of RNA biology.
PI: Carine TISNE, UMR 8261; tisne@ibpc.fr

Publications related to the project :
Oerum S. et al (2021), A comprehensive review of m6A/m6Am RNA methyltransferase structures. Nucleic Acids Res. 49(13):7239-7255. doi: 10.1093/nar/gkab378.
Oerum S, Dendooven T, Catala M, Gilet L, Dégut C, Trinquier A, Bourguet M, Barraud P, Cianferani S, Luisi BF, Condon C, Tisné C. (2020), Structures of B. subtilis Maturation RNases Captured on 50S Ribosome with Pre-rRNAs. Mol Cell. 80(2):227-236. doi: 10.1016/j.molcel.2020.09.008.
Barraud P, Gato A, Heiss M, Catala M, Kellner S, Tisné C. (2019), Time-resolved NMR monitoring of tRNA maturation. Nat Commun. 10(1):3373. doi: 10.1038/s41467-019-11356-w.

Activities

- Biochemistry (purification of proteins and RNA, in vitro transcription, preparation of RNA/protein complexes)
- Structural biology (NMR and/or X-ray crystallography and/or CryoEM)
- Enzymatic activity assays

Skills

Knowledge
- Expert in structural biology (CryoEM preferred)
- General knowledge in molecular biology (cloning, E. coli transformation, etc)
- General knowledge in biochemistry (enzymology, protein purification, etc)
- Fluent in English, good skill in scientific communication

● Know-how
- working with RNA and proteins
- processing of the structural data
- Plan and implement a scientific study
- Organise your work on a daily basis
- Keeping a laboratory notebook and presenting your results

● Soft skills
- Work in a team
- Open-minded, curious and good at synthesizing information
- Organised, like to do a good job and finish it well
- critical of results, honest

● Management skills
- Co-supervision of Master students possible

Work Context

All the experiments will be performed at the CNRS, at the Institute of Physical and Chemical Biology on the Curie campus, in the 5th district of Paris. All the necessary equipment is already in the laboratory or nearby.

The COFUND FP-DYNAMO-PARIS programme offers a unique interdisciplinary environment and a fundamental research initiative in the centre of Paris to train the scientists of tomorrow in the field of physico-chemical biology. The goal of the research programme is to integrate knowledge on gene expression, structural and membrane biology, and bioenergetics in bacteria, chloroplasts and mitochondria in the general context of improving our understanding the biogenesis of energy-transducing membranes. These studies will involve cutting-edge technologies including structural biology (NMR, X-ray crystallography and CryoEM), RNA sequencing, mass-spectrometry, synthetic biology, microfluidics, computational modelling and visualization. Post-doctoral fellows will have the opportunity of developing their research projects in a dynamic scientific environment covering three main research themes: i) RNA biology from bacteria to chloroplasts, ii) Membrane system dynamics and iii) computational modelling of molecular assemblies. They will benefit from the supervision of internationally-recognised experts in their fields, a world-wide network of renowned institutions, cutting-edge research infrastructure and state-of-the-art technical know-how that will allow them to hone their scientific skills, make themselves known and increase their competitiveness on the labour market. Specific training will be proposed to ERs through attendance of the EMBO course on “Laboratory Leadership for Postdocs” included in a retreat in Padua, and involvement in dedicated complementary soft skill training events.

Constraints and risks

Fellowship period should start between January 1 and May 31 2022.

Additional Information

The application should be written in English and uploaded at https://emploi.cnrs.fr/. It should include a detailed CV, a minimum of two letters of reference, a letter of motivation describing your short and long term career objectives, and a signed declaration stating that you are in compliance with the mobility rule which stipulates that you have not resided or carried out your main activity (work or studies) in France for more than 12 months during the three years preceding the date of recruitment. In addition, the complete file must also be sent to Bruno MIROUX & Magdalena TORTYNA, respectively director and manager of the project at the following email address DYNAMOcofund@ibpc.fr (Tel: 33 1 58 41 50 64). See details on the website http://labexdynamo.ibpc.fr/fp-dynamo-paris/
The position is intended as an initial step in a research career, and the assessment of the applicants will primarily be based on their research qualifications and their potential as researchers with skills that complement and strengthen on-going research within the partner institutions.

Step 1. The applications of each eligible candidate will be evaluated by independent international experts within the relevant research theme. Eligible applicants will be evaluated based on merits, motivation for the program, academic excellence and personal qualifications such as collaborative and communication skills.

Step 2. A maximum of three candidates will be retained for a video interview with the independent experts for each open position (Nov 2021). After the interview, candidates on the short list will be invited to a conference call with the project supervisor and a DYNAMO board member to define the general scope of the project, the training and the research environment. Candidates will be asked to draft a 3-page project proposal, bringing their own original ideas and will be given 2 weeks to submit it.

Step 3. Each project proposal will be evaluated by one of the independent experts and the DYNAMO board member who evaluated the candidate in the previous steps. The final selection will be made based on the scores obtained at steps 1, 2 and 3. The minimum score must be above 20/40. Top candidate will be offered the postdoc position with a starting date from January 1- May 31, 2022.

The CNRS (Centre National de la Recherche Scientifique) is an equal opportunity employer. Furthermore, FP-DYNAMO-PARIS is committed to promoting the role of women in science and thus explicitly encourages women to apply.

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