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Reference : UPR3212-JULQUI-014
Workplace : STRASBOURG
Date of publication : Thursday, July 15, 2021
Scientific Responsible name : Dr Alexandre Charlet
Type of Contract : PhD Student contract / Thesis offer
Contract Period : 36 months
Start date of the thesis : 1 October 2021
Proportion of work : Full time
Remuneration : 2 135,00 € gross monthly
Description of the thesis topic
Oxytocin, a hypothalamic peptide, has long been associated with maternal behavior with its implication in childbirth, breastfeeding or even mother-child attachment. Numerous studies now show that oxytocin is strongly involved in the control of many other physiological functions, ranging from complex sexual behaviors which have often earned it the nickname "molecule of love", to the complex regulation of emotions, including pain. Although the regulation of neural circuits by oxytocin has received much attention, the current dogma is that oxytocin, like other neuropeptides, acts in the central nervous system exclusively through neurons.
However, previous work by the team tends to show that the modulatory effect of oxytocin, both on the activity of neural networks and on the regulation of inherent behavior, is highly dependent on astrocytes.
The aim of this thesis will be to help elucidate the intracellular mechanisms responsible for the variations in astrocytic calcium induced by the activation of the oxytocin receptor. The results obtained will be compared with the role of the activity of the neurons of the central amygdala. To do this, we will use pharmacological and optogenetic approaches to which we will couple ex vivo recordings in patch clamp electrophysiology and calcium imaging.
The recruited candidate will join the Institute of Cellular and Integrative Neurosciences (INCI UPR 3212) in Strasbourg. INCI comprises 130 people and has 11 research teams. The doctoral student will join the Peptidergic Control of Emotions team composed of 6 people (including 2 researchers).
The doctoral student will be supervised by Dr Alexandre Charlet.
The Strasbourg Doctoral School of Life and Health Sciences ED414 is associated with this training.
Constraints and risks
Scheduled work to be expected.
The interested doctoral student must have a master's degree in neuroscience and ideally have a strong experience in ex vivo patch clamp, and / or in vivo calcium imaging is essential. The doctoral student must be familiar with analysis tools, and particularly Python code languages, is an undeniable advantage. A motivated mind, capable of analysis and scientific synthesis is necessary.
Applications should include a cover letter and CV, as well as the names and email addresses of two academic references.
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