Uncoupling Nitrogen Use Efficiency and response to drought by manipulating the CLCa vacuolar nitrate transporter in Arabidopsis (M/F)
New
- FTC PhD student / Offer for thesis
- 36 months
- BAC+5
This offer is available in English version
This offer is open to people with a document recognizing their status as a disabled worker.
Offer at a glance
The Unit
Institut de Biologie Intégrative de la Cellule
Contract Type
FTC PhD student / Offer for thesis
Working hHours
Full Time
Workplace
91198 GIF SUR YVETTE
Contract Duration
36 months
Date of Hire
01/01/2027
Remuneration
2300 € gross monthly
Apply Application Deadline : 25 November 2026 23:59
Job Description
Thesis Subject
A 36-month doctoral position is available at the Institute for Integrative Biology of the Cell (I2BC), located on the CNRS research campus in Gif-sur-Yvette, France. The successful candidate will join a project funded by the ANR (French Agency for research) and conducted in close collaboration with the Jean-Pierre Bourgin Institute (IJPB-INRAE, Versailles) and the Institute of Genetics for the Environment and Plant Protection (IGEPP-INRAE, Rennes).
This project aims to investigate the effects of altering vacuolar nitrate storage on nitrogen use efficiency (NUE), water use efficiency (WUE), and drought stress tolerance by studying the CLCa transporter in the model plant species Arabidopsis thaliana.
Responsibilities
Using integrated approaches combining plant physiology, molecular biology, and phenotyping, the the PhD candidate will investigate the impact of modifying nitrate storage in different organs, cell types, or developmental stages.
Main responsibilities will include:
• Designing molecular constructs, cloning, and preparing plasmids for the genetic transformation of Arabidopsis thaliana;
• Plant cultivation under controlled conditions (in vitro, substrate-based, and hydroponic systems);
• Analysis of traits related to nitrogen use efficiency (NUE), including nitrate, amino acid, and protein quantification, as well as the measurement of enzyme activities;
• Assessment of water use efficiency (WUE)-related parameters, including water content determination, gas exchange measurements, and stomatal aperture analyses;
• Development and implementation of phenotyping approaches to evaluate plant responses to nutritional and water stresses;
• Data analysis, visualization, and presentation during meetings with project collaborators;
• Dissemination of research findings through seminars, scientific conferences, and publications in peer-reviewed international journals.
Required qualifications
Applicants must hold a Master's degree (or equivalent) in plant biology and physiology or a related discipline. A strong background in plant nutritional physiology is required. Previous experience in plant molecular biology and physiology using the model species Arabidopsis thaliana, as well as in nitrogen physiology, would be an additional asset.
The successful candidate must be able to work effectively in a collaborative, multidisciplinary research environment to carry out the project. Proficiency in scientific English, both written and spoken, is required to present research findings, participate in consortium meetings, and interact effectively with the various project partners. Finally, the candidate must be willing to travel for research visits to partner institutes.
Your Work Environment
The PhD candidate will join the Integrated Approaches to Ion Transport team within the Cell Biology Department of the Institute for Integrative Biology of the Cell (I2BC), located on the CNRS research campus in Gif-sur-Yvette, France. The project will be carried out in close collaboration with the SATURNE team at IJPB (INRAE Versailles) and with the P2M2 team at IGEPP (INRAE Rennes), providing a highly complementary research environment at the interface of plant physiology, mineral nutrition, and cell biology.
The successful candidate will have access to state-of-the-art experimental facilities, including controlled growth chambers and fully equipped laboratories for molecular biology, biochemistry, and plant physiology. The I2BC team is member of the Saclay Plant Sciences (SPS) network, which brings together approximately 60 research groups. This unique environment offers numerous opportunities for interdisciplinary interactions, scientific collaborations, and professional development.
Constraints and risks
not applicable
Compensation and benefits
Compensation
2300 € gross monthly
Annual leave and RTT
44 jours
Remote Working practice and compensation
Pratique et indemnisation du TT
Transport
Prise en charge à 75% du coût et forfait mobilité durable jusqu’à 300€
About the offer
| Offer reference | UMR9198-SOPFIL-002 |
|---|---|
| CN Section(s) / Research Area | Integrative plant biology |
About the CNRS
The CNRS is a major player in fundamental research on a global scale. The CNRS is the only French organization active in all scientific fields. Its unique position as a multi-specialist allows it to bring together different disciplines to address the most important challenges of the contemporary world, in connection with the actors of change.
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