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Ph.D. Position Three-photon microscopy of brain and developing tissue

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Français - Anglais

Date Limite Candidature : vendredi 2 juillet 2021

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General information

Reference : UMR7645-CHRFRA-001
Workplace : PALAISEAU
Date of publication : Friday, June 11, 2021
Scientific Responsible name : BEAUREPAIRE Emmanuel
Type of Contract : PhD Student contract / Thesis offer
Contract Period : 36 months
Start date of the thesis : 1 October 2021
Proportion of work : Full time
Remuneration : 2 135,00 € gross monthly

Description of the thesis topic

Three-photon microscopy of brain and developing tissue
Two-photon microscopy uses a focused infrared femtosecond laser to record fluorescence images with 3D cellular resolution at depths reaching 300-500 µm inside scattering biological tissues. Thanks to these properties, it is a widely used technique in neuroscience and embryology. However, deeper imaging is very difficult because scattering degrades the image contrast. A promising recent technique to push this depth limit is based on three-photon (3P) excitation, which provides superior excitation confinement in the presence of scattering and makes it possible to reach depths exceeding 1 mm in brain tissue. This novel approach opens many application possibilities, and at the same time motivates technological developments and optimizations.
The Laboratory for Optics and Biosciences (LOB) at Ecole Polytechnique is actively developing 3P imaging, and has recently built a microscope prototype providing dual-color 3P imaging. The objective of this project will to implement advanced developments such as adaptive optics, multicontrast and multiplane imaging based on the existing setup, and to explore the application of 3P microscopy to in-depth and long-term imaging of developing tissues.
The work will be done in an interdisciplinary team at LOB and within the framework of ERC-funded European project. An ideal candidate has an engineering degree or Master's degree in optics, with skills in instrumentation and programming. Interest in biological and biomedical applications would be desirable. Previous experience in multiphoton microscopy or femtosecond lasers will be an asset.

Work Context

The project will be carried out in the Laboratory for Optics and Biosciences at Ecole Polytechnique (Palaiseau) in the advanced microscopies team, and will be supervised by Emmanuel Beaurepaire. The host team provides an interdisciplinary environment combining optics, computational science, and biology. The work will involve daily interactions with a group of ~3-4 people, within a local microscopy team of ~25 persons, and interactions with the ERC-SyG HOPE project collaborators (Institut de la Vision, Paris; INMED, Marseille). Ecole Polytechnique is located in the Paris-Saclay area, and is easily accessible by transport from Paris. More information available on https://www.ip-paris.fr. The candidate will join the doctoral school of the Institut Polytechnique de Paris, in the field of physics.

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