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PhD thesis offer Chemistry-biology interface, spectroscopy (M/F)

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Français - Anglais

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General information

Reference : UMR7313-THITRO-001
Workplace : MARSEILLE 13
Date of publication : Sunday, March 01, 2020
Scientific Responsible name : Thierry Tron
Type of Contract : PhD Student contract / Thesis offer
Contract Period : 36 months
Start date of the thesis : 1 October 2020
Proportion of work : Full time
Remuneration : 2 135,00 € gross monthly

Description of the thesis topic

Multi Copper Oxidases (MCO) are particularly robust biomolecules with distinctive spectroscopic characteristics behaving like a biological capacitor where up to 4 electrons and 4 protons are transiently stored. Within the framework of this project funded by the CNRS, our ambitious objective is to use light to trigger the transfer of electrons (ET) in an MCO from discrete graft points on the surface, this in order to study the ET intramolecular, the distribution of electrons on the 4 CuII ions and the mechanistic aspects of the reaction O2 + 4e- + 4H + <-> 2H2O (reversible). The system (a fungal laccase) will be studied with advanced spectroscopic techniques (UV / VIS, EPR, stopped-flow) and photolysis by laser flash. This project will benefit from the combined experience of two teams (CNRS and CEA) in: i) the production of laccase variants on a g.L-1 scale ensuring a comfortable physico-chemical characterization of enzymes; ii) modification of the enzyme and precise grafting of sensitizers / probes on the surface; iii) advanced photophysics and analysis of kinetic data; iv) the use of MCO as terminal oxidant in oxidative photocatalysis.

Work Context

The research work will mainly be carried out at the Institute of Molecular Sciences in Marseille (iSm2, UMR CNRS 7313, Aix Marseille University) within the BiosCiences team. This team is at the interface of chemistry and biology with varied skills ranging from chemistry, spectroscopy to biochemistry. Part of the work (laser flash photolysis) will be carried out at the Photocatalysis and Biohydrogen Laboratory, at the Institute of Biophysical and Structural Biology, UMR9198, CEA Saclay.

Additional Information

Highly motivated candidates (master level or equivalent with honors) with solid training in molecular chemistry and / or biological chemistry (chemistry-biology interface). Experience in spectroscopy, homogeneous catalysis (transition metals), or any other field related to the project would be an asset (non-discriminating criterion).

For any further information on the project and / or the recruitment process, contact Dr. Thierry Tron (thierry.tron@univ-amu.fr). Please note, no application by email will be considered (only via the CNRS job portal). All applications must be submitted via the CNRS job portal and must include a CV, a motivation letter, contact details for at least two references, as well as transcripts.

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