Reference : UMR7288-CHRESS-042
Workplace : MARSEILLE 09
Date of publication : Wednesday, September 14, 2022
Scientific Responsible name : J.ROYET
Type of Contract : PhD Student contract / Thesis offer
Contract Period : 36 months
Start date of the thesis : 1 January 2023
Proportion of work : Full time
Remuneration : 2 135,00 € gross monthly
Description of the thesis topic
Our body hosts billions of bacteria that colonize our skin our lungs and our gut. The beneficial effect of some of these microorganisms on the development and functioning of these bodies part has been well documented. Recent studies show that by acting on the nervous system, gut bacteria also called microbiota, can also change the behavior of infected individuals (4). However, the underlying cellular and molecular mechanisms remain mostly unknown. The laboratory of Julien Royet uses the Drosophila model to dissect the molecular dialog between the microbiota and the nervous system of the host. We have recently shown that infected flies have an altered behavior and demonstrated that bacterial peptidoglycan is changing the comportment of infected Drosophila by modulating NF-kB signaling in octopaminergic neurons (1,2,5). Using Drosophila genetics, CRISPR-mediated genome editing and latest imaging technologies, the recruited student will perform experiments to understand how bacteria modulates the activity of neurons and how this modulation translates into behavioural changes. Given the remarkable evolutionary conservation in host defense and neuronal mechanisms, we believe that the results obtained should be of significant relevance to an in-depth understanding of bacteria-neurons interactions in organisms other than Drosophila and namely in mammals in which such analyses are difficult to conduct.
Recent publications of the host lab:
1. Kurz CL, et. al. Peptidoglycan sensing by octopaminergic neurons modulates Drosophila oviposition. Elife. 2017 Mar 7;6. pii: e21937.
2. Charroux B, et al. Cytosolic and Secreted Peptidoglycan-Degrading Enzymes in Drosophila Respectively Control Local and Systemic Immune Responses to Microbiota. Cell Host Microbe. 2018 Feb 14;23(2):215-228.e4.
3. Masuzzo A, et al, Peptidoglycan-dependent NF-κB activation in a small subset of brain octopaminergic neurons controls female oviposition. Elife. 2019 Oct 29;8:e50559. doi: 10.7554/eLife.50559.
4. Masuzzo A, Montanari M, Kurz L, Royet J. How Bacteria Impact Host Nervous System and Behaviors: Lessons from Flies and Worms. Trends Neurosci. 2020 Dec;43(12):998-1010.
5. Charroux B, Daian F, Royet J. Drosophila Aversive Behavior toward Erwinia carotovora carotovora Is Mediated by Bitter Neurons and Leukokinin. iScience. 2020 Jun 26;23(6):101152. doi: 10.1016/j.isci.2020.101152.
6. Martina M and J Royet. Impact of Microorganisms and Parasites on Neuronally Controlled Drosophila Behaviours Cells 2021, 10(9), 2350; https://doi.org/10.3390/cells10092350
IBDM is located on the Luminy Campus and comprises about 240 permanent researchers, professors, engineers and technicians and non-permanent staff (CDD, post-docs, PhD students, trainees) distributed over 21 research teams and 11 technical platforms and services.
IBDM is a joint research unit under the supervision of the CNRS and AMU, which explores the field of developmental biology and associated pathologies.
The activity will be carried out within the team " Host pathogen interaction in the Drosophila model" directed by Julien ROYET
As the team is composed of several nationalities, the practice of the English language is essential.
Constraints and risks
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