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Reference : UMR7288-CATCOR-029
Workplace : MARSEILLE 09
Date of publication : Wednesday, November 21, 2018
Scientific Responsible name : Cedric MAURANGE
Type of Contract : PhD Student contract / Thesis offer
Contract Period : 36 months
Start date of the thesis : 1 February 2019
Proportion of work : Full time
Remuneration : 1 768,55 € gross monthly
Description of the thesis topic
The mechanisms that promote and limit the proliferation of the different types of tissue stem cells during embryonic and fetal development are still poorly understood. Yet understanding these mechanisms is essential to better control stem cell proliferation for regenerative therapy, as well as to identify new ways to block the proliferation of cancer stem cells.
Our lab uses the fruit fly Drosophila to identify evolutionary conserved mechanisms, that control the proliferation of stem cells. Recently, our work has highlighted a transcription factor, Chinmo, that is expressed in stem cells of different tissues during early Drosophila development and that promotes their proliferation (Narbonne-Reveau et al. 2016; Dillard et al. 2018). We have also been able to demonstrate that the deregulation of chinmo can induce tumors while its controlled expression can increase the regenerative potential of damaged tissues. However, the mode of action of this transcription factor and its target genes are unknown.
The PhD student will have the task of identifying the direct target genes of Chinmo in order to understand how Chinmo imposes a proliferative state on stem cells during development and tumorigenesis, making them resistant to differentiation. A study of the chromatin status of these genes will also be carried out to study whether Chinmo is able to format the chromatin landscape of stem cells.
This work will provide a better understanding of the mechanisms defining a stem cell state during early development and tumorigenesis.
- The PhD student will use the TaDa technique (targeted DamID) which makes it possible to identify the binding sites of a transcription factor in a particular cell type.
- He/she will also implement the ATAC-seq technique to determine the chromatin status of target genes.
In order to implement these technologies, the student will routinely perform dissections of the Drosophila central nervous system, immunolabeling and confocal microscopy, learn to use bioinformatics tools such as R, maintain and manage stocks of Drosophila lines, collect results, format them and present them regularly in English at lab meetings.
The PhD student will be supervised by Dr Cédric Maurange, head of the "Neural Stem Cell Plasticity" team (http://www.ibdm.univ-mrs.fr/equipe/neural-stem-cell-plasticity/) at the Developmental Biology Institute of Marseille (IBDM).
The IBDM, located on the Luminy Campus, includes approximately 240 full time researchers, teacher-researchers, engineers and technicians and non-permanent CDDs, post-docs, doctoral students, trainees spread over 21 research teams and 11 technical platforms and services. The IBDM is a joint research unit under the supervision of the CNRS and AMU, which explores the field of developmental biology and associated pathologies.
SKILLS REQUIRED :
- Ability to work in a team environment
- Good interpersonal skills
- Strong knowledge of molecular biology
- Good command of English: written and spoken
- Good ability to adapt to new techniques
Constraints and risks
Possible variability of working hours
Please post you CV, cover letter and references
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