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Doctorant H/F in biology

This offer is available in the following languages:
Français - Anglais

Date Limite Candidature : mardi 8 décembre 2020

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General information

Reference : UMR7267-FRERIC-001
Workplace : POITIERS
Date of publication : Tuesday, November 17, 2020
Scientific Responsible name : Freddie-Jeanne Richard
Type of Contract : PhD Student contract / Thesis offer
Contract Period : 36 months
Start date of the thesis : 1 February 2021
Proportion of work : Full time
Remuneration : 2 135,00 € gross monthly

Description of the thesis topic

Wild and domestic bees are necessary for ecosystems functioning and for the agricultural production of many vegetables and fruits. The honeybee, whose economic impact linked to its action is considerable, has been in danger for several years. Pests (viruses, bacteria, parasites) and chemical compounds (pesticides) tested individually do not explain alone the sharp decline in bee populations. However, new insecticides are put on the market regularly without studies on the sublethal effects which can have a strong impact on the colony. As part of our project we want to measure the impact of pesticides from laboratory tests on larvae and in situ on adults in semi-natural conditions. The mortality and life history traits of the workers over their entire cycle, larval and adult, duration of development, as well as social interactions will be assessed.

This thesis will require the use and development of skills in the fields of ecology, behavior, ecotoxicology, molecular biology, and chemical ecology. It will be carried out in collaboration between researchers belonging to laboratories under the supervision of CNRS, INRAe and IRD.
This thesis subject is funded within the framework of an Ecophyto II + call and the funding will cover the expenses related to the realization of the experiments carried out during the thesis as well as to the valorization of the results by participating in scientific events ( congress) and at the cost of publication of the results.

References
Aupinel P, et al. 2007. A new larval in vitro rearing method to test effects of pesticides on honey bee brood. Redia. 90: 91-94.
Decourtye A, et al. 2009. Sublethal effects of fipronil on the ability of honeybees (Apis mellifera L.) to orientate in a complex maze. Julius-Kühn-Arch. 423: 75-83.
Fourrier J, et al. 2015. Larval exposure to the juvenile hormone analog pyriproxyfen disrupts acceptance and Social behavior performance in adult honeybees. Plos One. 10(7): e0132985
Henry M, et al. 2012. A common pesticide decreases foraging success and survival in honeybees. Science. 336(6079): 348-350.

Papach A, et al. 2017. Thiamethoxam and A. Foulbrood: in vitro effects on mortality & cognition of honey bee. J Apic Res. 56(4): 475-486.

Richard FJ, et al. 2012. Effects of immunostimulation on social behavior, chemical communication and genome-wide gene expression in honey bee workers (Apis mellifera). BMC Genomics. 13: 558
Richard F-J, et al. 2008. Modulation of social interactions by immune stimulation in honey bee workers, Apis mellifera. BMC Biology. 6: 50.

Work Context

The doctoral student is recruited by the Centre National pour la Recherche Scientifique - CNRS . The EXPLORA scientific project in which the thesis is part is funded by the ECOPHYTO II + plan co-piloted by the ministries of Agriculture and Food, Ecological and Solidarity Transition, Solidarity and Health and the Ministry of Higher Education, Research and Innovation as well as by the French Office for Biodiversity.
The thesis will take place on two sites (1) at the Ecology and Biology of Interactions Laboratory, UMR CNRS (7267) and University of Poitiers based in Poitiers and (2) at the APIS unit of INRAe du Magneraud (17700 Surgères) .
The doctoral student will be under the responsibility of the leader of the Explora project based at the EBI laboratory on the science campus of the University of Poitiers and under the responsibility of the director of the APIS unit at INRAe du Magneraud.
The EBI laboratory is divided in three teams and the doctoral student will be attached to the Ecology Evolution Symbiose team made up of 23 permanent staff and 11 contractual staff. The EBI laboratory has numerous molecular biology equipment, chromatography, behavioral analysis tools, etc. The student will also benefit on the INRAe site from the support of the members of the unit and the apiary as well as the training necessary for carrying out the various experiments (larval rearing, RFID, etc.).
The student will be able to present her/his work in various congresses (international and national).
The candidate will be registered at the Théodore Monod doctoral school (https://www.u-ldevinci.fr/cega/) de l'Université de Poitiers.

Constraints and risks

Field experiments (spring and summer) will take place on the INRAe site and laboratory experiments at EBI (fall and winter). This location on two sites per period of 6 months will induce accommodation constraints at the expense of the doctoral student. On the other hand, travel to these two sites made on an ad hoc basis will be covered by the program (for example for meetings).
In order to limit travel and for experimental constraints, the doctoral student will have to follow the doctoral school course between September and March.
The candidate must be trained in handling live animals (bees) and not have any known allergy to bee stings.

Additional Information

Master's level (or equivalent) in biology with skills in beekeeping and / or chemical ecology and / or molecular biology. The thesis subject being multidisciplinary, skills or affinity in the fields of competence indicated will be a plus.
The candidate should have a strong interest in bees, fieldwork, bench-top, data analysis skills, scientific writing (exclusively in English).
Curiosity, rigor, autonomy and communication skills are important assets.
Only candidates with an MSc degree can apply. The international services of the University of Poitiers are very active and can provide additional support for the visa application or specific support for international applicants. More information on our campus: https://www.univ-poitiers.fr/en/live-campuses/
The deadline for receipt of applications is December 7, 2020

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