Ph.D. student in analytical chemistry specializing in protein analysis using structural mass spectrometry technique (M/F)

Job Description

Thesis Subject

Surfing on the catalytic mechanism of the IspG metalloenzyme with innovative molecular tools

To develop analytical approaches based on native mass spectrometry in combination with high-resolution ion mobility spectrometry (nIM-MS), and hydrogen-deuterium exchange mass spectrometry (HDX-MS) to decipher the inhibition mechanism of IspG.
The doctoral student's responsibilities will include the following:
- Development of native MS approaches in direct infusion or in combination with non-denaturing LC methods
- Optimization of nIM-MS approaches in classical and high-resolution ion mobility devices
- Implementation of collision induced unfolding (CIU) to evaluate IspG protein gas-phase stability upon interaction with the different inhibitors.
-Development of HDX-MS methods to evaluate the impact of the new inhibitors in IspG conformation and dynamics.
- Bioinformatics interpretation of data.
- Monitor project(s) and participate in collaboration meetings.
- Write reports.
- Present, disseminate and promote the results obtained in the frame of the project.
- Use and routine maintenance of mass spectrometry instruments.
- Ensure traceability of analyses as part of LSMBO's quality approach (ISO9001 environment).

Your Work Environment

The PhD will be attached to the Doctoral School of Chemical Sciences of the University of Strasbourg (ED222).

The IPHC, a joint research unit jointly supervised by the CNRS and the University of Strasbourg (UMR7178), is a multidisciplinary laboratory where research teams from different scientific backgrounds (ecology, physiology and ethology, chemistry, and subatomic physics) develop high-level research programs based on scientific instrumentation. The IPHC is organized into three departments and has a total staff of 393 employees (M/F), including 257 permanent staff (119 researchers and faculty members and 138 engineers and technicians, M/F), 46 employees on fixed-term contracts, and 102 doctoral students (M/F).
The PhD student will join the LSMBO (Laboratory of Bioorganic Mass Spectrometry) team at the IPHC, which consists of 12 researchers and faculty members and 9 permanent engineers and technicians (M/F)
The PhD student will interact on a daily basis with all LSMBO staff, including mass spectrometry engineers, computer scientists and bioinformaticians. He or she will also interact with collaborators from outside the laboratory.
He or she must demonstrate a solid understanding of :
- Advanced knowledge in bioanalytical chemistry, mass spectrometry, and ion mobility.
- Knowledge in native MS, and HDX-MS
- Knowledge in non-denaturing LC coupled to nMS.
- Knowledge in CIU experiments with different experimental configurations.

The doctoral student must be able to communicate in written and spoken English at the B2 level, as defined by the Common European Framework of Reference for Languages, in order to draft regular reports and present findings to English-speaking colleagues.
Applications should include a detailed CV, a letter of motivation, a summary of the Master's thesis as well as the grades of Master 1 and 2.

Constraints and risks

-Casual work in staggered schedule
-Work in an ISO 9001 certified environment

Compensation and benefits

Compensation

2300 € gross monthly

Annual leave and RTT

44 jours

Remote Working practice and compensation

Pratique et indemnisation du TT

Transport

Prise en charge à 75% du coût et forfait mobilité durable jusqu’à 300€

About the offer

About the CNRS

The CNRS is a major player in fundamental research on a global scale. The CNRS is the only French organization active in all scientific fields. Its unique position as a multi-specialist allows it to bring together different disciplines to address the most important challenges of the contemporary world, in connection with the actors of change.

CNRS

The research professions